Confocal, three-dimensional tracking of individual quantum dots in high-background environments.
Confocal, three-dimensional tracking of individual quantum dots in high-background environments.
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DOI：
10.1021/ac8021899
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发表时间：
2008-12-15
影响因子：
7.4
通讯作者：
Werner, James H.
中科院分区：
文献类型：
--
作者：
Wells, Nathan P.;Lessard, Guillaume A.;Werner, James H.
We demonstrate a custom confocal fluorescence-microscope that is capable of tracking individual quantum dots undergoing three dimensional Brownian motion (diffusion coefficient ~0.5 µm2/s) in environments with a signal-to-background ratio as low as 2:1, significantly worse than observed in a typical cellular environment. By utilizing a pulsed excitation source and time-correlated single photon counting, the time-resolved photon stream can be used to determine changes in the emission lifetime as a function of position and positively identify single quantum dots via photon-pair correlations. These results indicate that this microscope will be capable of following protein and RNA transport throughout the full 3 dimensional volume of a live cell for durations up to 15 seconds.
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