Autoantibodies to the proteasome in monosymptomatic optic neuritis may predict progression to multiple sclerosis
Autoantibodies to the proteasome in monosymptomatic optic neuritis may predict progression to multiple sclerosis
复制标题
DOI：
10.1080/00365510701342062
复制
发表时间：
2007-01-01
影响因子：
2.1
通讯作者：
Frederiksen, J. Lautrup
中科院分区：
文献类型：
--
作者：
Beyer, N. H.;Milthers, J.;Frederiksen, J. Lautrup
目标。在多发性硬化症（MS）患者血清和脑脊液（CSF）中发现了蛋白酶体自身抗体（PAB）。因此，PAB的存在可能是多发性硬化症的诊断标志物。我们研究了急性单症状视神经炎（ON）和多发性硬化症患者的PAB血清状态是否与健康对照组有显著差异，以及PAB状态是否可预测ON患者后期多发性硬化症的发展。材料和方法。采用ELISA对ON患者、MS患者和健康供者血清进行回顾性分析。随后，一小部分阳性样本进行SDS-PAGE、免疫印迹和2-D PAGE。结果。我们发现20%（6/30）的ON患者、47%（22/47）的MS患者和9%（7/81）的对照患者采用ELISA检测PAB阳性。在2例（4%）MS患者、1例（3%）ON患者和2例（3%）对照中发现高PAB水平。免疫印迹法证实ELISA检测PAB阳性。用2D PAGE分离蛋白酶体亚基，然后进行免疫印迹，结果显示没有特定的PAB亚基偏好。结论。对现有患者档案的回顾性检索显示，6例（100.0%）ab阳性ON患者中有6例随着时间的推移发展为MS。24例PAB阴性ON患者中有8例（33%）随着时间的推移发展为多发性硬化症，47%（14/30）的ON患者发展为多发性硬化症。通过ELISA分析一系列患者CSF以评估并发血清PAB状态与CSF样本之间可能的相关性，但未发现相关性。然而，来自6名ab阳性ON患者的结果可能具有潜在的预后价值。
Objective. Proteasome autoantibodies (PAB) have been found in multiple sclerosis (MS) patient sera and cerebrospinal fluid (CSF). Presence of PAB could thus be a possible diagnostic marker for MS. We investigated whether PAB serum status in acute monosymptomatic optic neuritis (ON) and MS differed significantly from that of healthy controls, and whether or not PAB status is predictive of later MS development in patients with ON. Material and methods. Sera from ON patients, MS patients and healthy donors were analysed retrospectively using ELISA. Subsequently, a small group of PAB-positive samples were subjected to SDS-PAGE, immunoblotting and 2-D PAGE. Results. We found that 20% (6/30) ON patients, 47% (22/47) MS patients and 9% (7/81) controls tested PAB positive using ELISA analysis. High PAB levels were found in 2 (4%) MS patients, 1 (3%) ON patient and 2 (3%) controls. PAB positivity in ELISA was confirmed by immunoblotting. Separation of proteasome subunits by 2D PAGE followed by immunoblotting revealed no particular PAB subunit preference. Conclusions. A retrospective search in available patient files revealed that 6 of 6 (100.0%) PAB-positive ON patients developed MS over time. Eight of 24 (33%) PAB-negative ON patients developed MS over time and 47% (14/30) of all ON patients developed MS. A series of patient CSF was analysed by ELISA to assess the possible correlation between PAB status of concurrent serum and CSF samples, but no correlation was found. However, the results from the six PAB-positive ON patients could potentially be of prognostic value.