Antifungal activities of Bacillus velezensis FJAT‐52631 and its lipopeptides against anthracnose pathogen Colletotrichum acutatum
Antifungal activities of Bacillus velezensis FJAT‐52631 and its lipopeptides against anthracnose pathogen Colletotrichum acutatum
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DOI：
10.1002/jobm.202200489
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发表时间：
2023-01
影响因子：
3.1
通讯作者：
Ying-Jie Deng;Zheng Chen;C. Ruan;R. Xiao;Heng-Ping Lian;Bo Liu;Mei-chun Chen;Jieping Wang
中科院分区：
文献类型：
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作者：
Ying-Jie Deng;Zheng Chen;C. Ruan;R. Xiao;Heng-Ping Lian;Bo Liu;Mei-chun Chen;Jieping Wang
本研究旨在研究Velezensis FJAT-52631及其脂肽对炭疽菌（Colletotrichum acutatum）的离体和原位抑菌活性。结果表明，菌株FJAT-52631及其脂肽粗品（10 mg/ml）对C. acutatum FJAT-30256的抑菌率为75.3%，抑菌圈直径为17.66 mm。FJAT-52631的活菌培养物和脂肽均能使枇杷炭疽病的发病延迟1 d，并降低枇杷炭疽病的原位发病率。B. Velezensis FJAT-52631在接种后第4天对枇杷炭疽病的防治效果为50%，而粗脂肽对枇杷炭疽病的防治效果不明显。全培养物处理组的平均病变直径为5.62 mm，小于对照组（6.81 mm）。FJAT-52631菌株分泌的伊枯草菌素A、芬枯草菌素A和表面活性素A等3种脂肽均具有抗真菌活性。其中，表面活性素A在1.25 mg/mL的浓度下显示出比其他两种脂肽更高的抗真菌活性，即使在60 mg/mL的浓度下。因此，FJAT-52631产生的表面活性素A在枇杷炭疽病的生防中起主要作用。扫描电子显微镜（SEM）观察表明，该菌菌丝体结构畸形。acutatum。以上结果表明，B. velezensis FJAT-52631对枇杷炭疽病具有较好的生防效果。acutatum。
This study was aim at investigating antifungal activities of Bacillus velezensis FJAT‐52631 and its lipopeptides against Colletotrichum acutatum ex situ and in situ. The results showed that the strain FJAT‐52631 and its crude lipopeptides (10 mg/ml) exhibited strong inhibitory effects on growth of C. acutatum FJAT‐30256 with an inhibition rate of 75.3% and an inhibition zone diameter of 17.66 mm, respectively. Both the viable bacterial cultures and lipopeptides of FJAT‐52631 could delay the onset of loquat anthracnose by 1 day and lower the incidence of loquat anthracnose in situ. The whole cultures of B. velezensis FJAT‐52631 displayed a 50% biocontrol efficacy on loquat anthracnose at the fourth day after inoculation, but the crude lipopeptides not. The average lesion diameter of the whole‐culture treated group was 5.62 mm, which was smaller than that of control group (6.81 mm). All the three types of lipopeptides including iturin A, fengycin, and surfactin A secreted from the strain FJAT‐52631 exhibited antifungal activities. Among them, surfactin A displayed higher antifungal activity at a concentration of 1.25 mg/mL than other two lipopeptides even if at a concentration of 60 mg/mL. Thus, the results indicated that surfactin A produced by FJAT‐52631 played a major role in the biocontrol of the loquat anthracnose. Scanning electron microscopy (SEM) observation revealed the structural deformities in the mycelia of C. acutatum. The above results suggested that the antifungal lipopeptides from B. velezensis FJAT‐52631 would be potential in biocontrol against anthracnose disease of loquat caused by C. acutatum.