The Zebrafish fgf Family
The Zebrafish fgf Family
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DOI：
10.1089/zeb.2007.0509
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发表时间：
2007-01-01
期刊：
影响因子：
2
通讯作者：
Konishi, Morichika
中科院分区：
文献类型：
--
作者：
Itoh, Nobuyuki;Konishi, Morichika
成纤维细胞生长因子（FGF）是分泌的多肽生长因子。FGF信号系统在脊椎动物的多种发育过程中起着至关重要的作用。人FGF家族由22个成员组成。虽然已经报道了16种斑马鱼fgf，但是斑马鱼fgf家族还没有得到很好的阐明。通过在斑马鱼基因组和cDNA数据库中进行基于同源性的搜索，我们已经确定了11个额外的斑马鱼fgf。斑马鱼fgf家族现在至少有27个成员。通过系统发育和基因定位分析，我们研究了斑马鱼FGF基因与人类FGF基因的关系。除FGF 9外，所有人FGF的斑马鱼直系同源物都已鉴定。斑马鱼的fgf9可能在进化过程中从基因组中丢失。此外，已经鉴定了斑马鱼fgf基因的6个旁系同源基因。系统发育分析表明，斑马鱼fgf基因家族可分为7个亚家族。斑马鱼FGF亚家族基本上与人类FGF亚家族一致，尽管有些包括潜在的旁系同源物。由于斑马鱼系统已被证明可用于研究基因功能和遗传疾病，因此本研究结果将有助于阐明FGF在斑马鱼和人类中的作用。
Fibroblast growth factors (FGFs) are secreted polypeptide growth factors. The FGF signaling system plays crucial roles in multiple developmental processes in vertebrates. The human FGF family comprises 22 members. Although 16 zebrafish fgfs have been reported, the zebrafish fgf family has not been well elucidated. We have identified 11 additional zebrafish fgfs by conducting a homology-based search in the zebrafish genome and cDNA databases. The zebrafish fgf family now comprises at least 27 members. By conducting phylogenetic and gene location analyses, we examined relationships of zebrafish fgf genes with human FGF genes. All the zebrafish orthologs of human FGFs except for FGF9 have been identified. Zebrafish fgf9 might have been lost from the genome during evolution. In addition, six paralogs of zebrafish fgf genes have been identified. The phylogenetic analysis suggests that the zebrafish fgf gene family can be divided into seven subfamilies. The zebrafish fgf subfamilies are essentially consistent with the human FGF subfamilies, although some include potential paralogs. As the zebrafish system has proved useful for studying gene functions and genetic diseases, the present findings will be useful for elucidation of roles of FGFs in zebrafish and humans.