HIGH-PERFORMANCE ELECTROPHORESIS - THE ELECTROPHORETIC COUNTERPART OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
HIGH-PERFORMANCE ELECTROPHORESIS - THE ELECTROPHORETIC COUNTERPART OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
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DOI：
10.1016/s0021-9673(01)96347-2
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
HJERTEN, S
中科院分区：
文献类型：
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作者：
HJERTEN, S
高效电泳是高效液相色谱的电泳等价物。获得非常有效的冷却，因为实验是在小直径（0.05-0.3毫米），壁厚仅为0.1-0.2毫米的玻璃管进行。在这些条件下，即使在高场强下，区域的热变形也很小，这使得分辨率高，运行时间短。当溶质通过自由区带电泳或凝胶扫描装置的固定UV检测器电泳迁移时，将溶质直接记录在玻璃管中。因此，不会因耗时的衍生化（染色）而导致检测延迟。电泳可以在筛分和非筛分凝胶中进行，也可以在无载体溶液中进行。分析，其可以包括低分子量化合物、蛋白质、核酸、病毒等，目前需要10-60分钟。通过使用液体冷却的电泳管，可以大大缩短该时间。对于蛋白质，约0.01-1 μg材料是合适的。
High-performance electrophoresis is the electrophoretic equivalent of high-performance liquid chromatography. Very efficient cooling is obtained because the experiments are performed in glass tubes of small diameter (0.05–0.3 mm) with a wall thickness of only 0.1–0.2 mm. Under these conditions the thermal deformation of a zone is small even at high field strengths, which allows high resolution and short run times. The solutes are recorded directly in the glass tube as they migrate electro-phoretically through the stationary UV detector of a free-zone electrophoresis or gel-scanning apparatus. Accordingly there is no delay in the detection caused by time-consuming derivatization (staining).The electrophoresis can be performed both in sieving and non-sieving gels and also in carrier-free solution. An analysis, which may comprise low-molecular-weight compounds, proteins, nucleic acids, viruses, etc., takes at present 10–60 min. This time can be considerably reduced by using a liquid-cooled electrophoresis tube. About 0.01–1 μg of material is suitable in the case of proteins.