Crystallization and preliminary X-ray analysis of the unliganded recombinant catalytic subunit of cAMP-dependent protein kinase.
Crystallization and preliminary X-ray analysis of the unliganded recombinant catalytic subunit of cAMP-dependent protein kinase.
复制标题
cAMP 依赖性蛋白激酶未配体重组催化亚基的结晶和初步 X 射线分析。
DOI：
10.1107/s0907444998005770
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Taylor,SS
中科院分区：
文献类型：
--
作者：
Narayana,N;Akamine,P;Xuong,NH;Taylor,SS
X-ray diffraction-quality crystals of the unliganded mouse recombinant catalytic subunit of cAMP-dependent protein kinase were grown by the hanging-drop vapour-diffusion technique using 2-methyl-2,4-pentanediol as precipitant. The crystals belong to the monoclinic space group P21 with unit-cell parameters a = 48.9, b = 147.4, c = 54.2 Å, β = 110.2°. A data set to 3.0 Å resolution with 92% completeness has been collected using synchrotron radiation. The unit cell contains four molecules of molecular weight 40 kDa with a corresponding volume solvent content of 45%.
