Study on Free Lipase-Catalyzed Ethanolysis for Biodiesel Preparation in an Oil/Water Biphasic System
Study on Free Lipase-Catalyzed Ethanolysis for Biodiesel Preparation in an Oil/Water Biphasic System
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DOI：
10.1007/s11746-011-1813-0
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发表时间：
2011-04
期刊：
影响因子：
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通讯作者：
H. Ren;Wei Du;L. Lv;De‐hua Liu
中科院分区：
文献类型：
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作者：
H. Ren;Wei Du;L. Lv;De‐hua Liu
游离脂肪酶作为生物柴油的催化剂具有成本低、反应速度快等优点，近年来受到广泛关注。我们前期的研究表明，游离脂肪酶NS81006可以有效地催化可再生油脂的甲醇分解。从可再生生物质中提取的乙醇具有更大的生产生物柴油的潜力。本文首次探索了乙醇作为脂肪酶催化制备生物柴油的酰基受体。系统研究了搅拌速率、含水量、醇油摩尔比和乙醇添加方式对脂肪酶NS81006催化乙醇解反应的影响。在最佳反应条件下，乙酯收率可达90%。进一步的研究表明，游离脂肪酶可以通过简单的油水相分离而重复使用，重复使用5次后，脂肪酶活力无明显损失。
Using free lipase as the catalyst for biodiesel production has drawn increasing attention in recent years due to its advantages of lower cost and faster reaction rate compared to immobilized lipase. Our previous study showed that free lipase NS81006 could effectively catalyze the methanolysis of renewable oil. Ethanol, derived from renewable biomass, has a greater potential for biodiesel production. In this paper, ethanol was explored for the first time as the acyl acceptor for free lipase‐mediated biodiesel preparation. The effect of stirring rate, water content, molar ratio of ethanol to oil and ethanol adding strategy was investigated systematically during the process of free lipase NS81006‐catalyzed ethanolysis. An ethyl ester yield of 90% was obtained under the optimized conditions. Further study showed that the free lipase could be repeatedly used by simple separation of the water phase from the oil phase and there was no obvious loss in lipase activity after five repeated uses.