Background correction in rapid scan EPR spectroscopy
Background correction in rapid scan EPR spectroscopy
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DOI：
10.1016/j.jmr.2018.05.010
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发表时间：
2018-08-01
影响因子：
2.2
通讯作者：
Eaton, Gareth R.
中科院分区：
文献类型：
--
作者：
Buchanan, Laura A.;Woodcock, Lukas B.;Eaton, Gareth R.
在快速扫描 EPR 中，快速变化的磁场会产生可能大于 EPR 信号的背景信号。已经开发出一种方法，通过采集两组数据（表示为扫描 1 和扫描 2）来校正背景信号。在扫描 2 中，外部场 B-0 反转，并且数据采集触发相对于扫描 1 中使用的设置偏移扫描场的半个周期。对于使用交叉环路谐振器采集的数据，从扫描 1 中减去扫描 2 可消除背景并增强 EPR 信号。实验在约 258 MHz 的 EPR 频率下进行，该频率处于体内成像常用的范围内。样品包括硝基氧自由基、三苯甲基自由基、二硝基氧和存在磁场梯度的硝基氧。该方法的优点是不对背景信号的形状做出任何假设，并且它提供了一种自动背景校正的方法。 (C) 2018 Elsevier Inc. 保留所有权利。
In rapid scan EPR the rapidly-changing magnetic field induces a background signal that may be larger than the EPR signal. A method has been developed to correct for that background signal by acquiring two sets of data, denoted as scan 1 and scan 2. In scan 2 the external field B-0 is reversed and the data acquisition trigger is offset by one half cycle of the scan field relative to the settings used in scan 1. For data acquired with a cross-loop resonator subtraction of scan 2 from scan 1 cancels the background and enhances the EPR signal. Experiments were performed at an EPR frequency of about 258 MHz, which is in the range that is commonly used for in vivo imaging. Samples include nitroxide radicals, a trityl radical, a dinitroxide, and a nitroxide in the presence of a magnetic field gradient. This method has the advantage that no assumption is made about the shape of the background signal, and it provides an approach to automating the background correction. (C) 2018 Elsevier Inc. All rights reserved.