Necdin mediates skeletal muscle regeneration by promoting myoblast survival and differentiation
Necdin mediates skeletal muscle regeneration by promoting myoblast survival and differentiation
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DOI：
10.1083/jcb.200701027
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发表时间：
2007-10-22
影响因子：
7.8
通讯作者：
Brunelli, Silvia
中科院分区：
文献类型：
--
作者：
Deponti, Daniela;Francois, Stephanie;Brunelli, Silvia
在病理性肌肉退化或受伤后丢失的肌纤维的再生通过新肌纤维的产生来维持。参与肌肉再生的一种重要细胞类型是卫星细胞。 Necdin 是一种在围产期生长期间在卫星细胞衍生的肌原性前体中表达的蛋白质。然而，其在肌生成中的功能尚不清楚。我们将骨骼肌中过度表达 necdin 的转基因小鼠与野生型和 necdin 缺失小鼠进行比较。肌肉损伤后，necdin 缺失小鼠在肌肉愈合方面表现出相当大的缺陷，而过度表达 necdin 的小鼠则显示出肌纤维再生的显着增加。我们还发现，在肌肉中，necdin 会增加肌细胞生成素的表达，加速分化，并抵抗成肌细胞凋亡。总的来说，这些数据阐明了 necdin 在骨骼肌中的功能和机制，并显示了 necdin 在肌肉再生中的重要性。
Regeneration of muscle fibers that are lost during pathological muscle degeneration or after injuries is sustained by the production of new myofibers. An important cell type involved in muscle regeneration is the satellite cell. Necdin is a protein expressed in satellite cell - derived myogenic precursors during perinatal growth. However, its function in myogenesis is not known. We compare transgenic mice that overexpress necdin in skeletal muscle with both wild- type and necdin null mice. After muscle injury the necdin null mice show a considerable defect in muscle healing, whereas mice that overexpress necdin show a substantial increase in myofiber regeneration. We also find that in muscle, necdin increases myogenin expression, accelerates differentiation, and counteracts myoblast apoptosis. Collectively, these data clarify the function and mechanism of necdin in skeletal muscle and show the importance of necdin in muscle regeneration.