Tricin inhibits CCL5 induction required for the efficient growth of human cytomegalovirus
Tricin inhibits CCL5 induction required for the efficient growth of human cytomegalovirus
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Tricin 抑制人巨细胞病毒有效生长所需的 CCL5 诱导
DOI：
10.1111/1348-0421.12590
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发表时间：
2018
影响因子：
2.6
通讯作者：
Tsugiya Murayama
中科院分区：
文献类型：
--
作者：
Akimasa Itho;Hidetaka Sadanari;Masaya Takemoto;Keiko Matsubara;Tohru Daikoku;Tsugiya Murayama
据报道，在感染人巨细胞病毒（HCMV）后，用麦黄酮（4′，5，7-三羟基-3 ′，5′-二甲氧基黄酮）处理人胚肺成纤维细胞（HEL）细胞可显著抑制HCMV复制。在本工作中，研究了麦黄酮在HEL细胞中抗HCMV作用的机制。结果发现，HEL细胞暴露于麦黄酮抑制HCMV复制，伴随着CC趋化因子RANTES（CCL 5）编码基因的转录物数量和CCL 5蛋白表达的减少。研究还发现，HCMV立即早期1（IE 1）和HCMV UL 54（编码DNA聚合酶）的转录物和HCMV的复制在CCL 5基因敲除细胞中显著降低。这些结果表明，麦黄酮的抗HCMV活性与更昔洛韦不同，并且CCL 5是参与HCMV复制的趋化因子之一。此外，趋化因子CCL 5可能是麦黄酮的靶点之一。
Treatment of human embryonic lung fibroblast (HEL) cells with tricin (4′, 5, 7‐trihydroxy‐3′, 5′‐dimethoxyflavone) following infection with human cytomegalovirus (HCMV) reportedly significantly suppresses HCMV replication. In the present work, the mechanisms for the anti‐HCMV effects of tricin in HEL cells were examined. It was found that exposure of HEL cells to tricin inhibited HCMV replication, with concomitant decreases in amounts of transcripts of the CC chemokine RANTES (CCL5)‐encoding gene and in expression of the CCL5 protein. It was also found that transcripts of HCMV immediate early 1 (IE1), and HCMVUL54(encoding DNA polymerase) and replication of HCMV was significantly lower inCCL5gene‐knockdown cells. These results suggest that the anti‐HCMV activity of tricin differs from that of ganciclovir and that CCL5 is one of the chemokines involved in HCMV replication. In addition, it is possible that chemokine CCL5 is one of the targets of tricin.