Modification of behavioral and neural taste responses to NaCl in C57BL/6 mice: effects of NaCl exposure and DOCA treatment.
Modification of behavioral and neural taste responses to NaCl in C57BL/6 mice: effects of NaCl exposure and DOCA treatment.
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C57BL/6 小鼠对氯化钠的行为和神经味觉反应的改变：氯化钠暴露和 DOCA 治疗的影响。
DOI：
10.1016/s0031-9384(98)00239-x
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发表时间：
1999
影响因子：
2.9
通讯作者：
Beauchamp,GK
中科院分区：
文献类型：
--
作者：
Bachmanov,AA;Inoue,M;Tordoff,MG;Ninomiya,Y;Beauchamp,GK
为了研究外周味觉反应对氯化钠接受度变化的可能作用，我们研究了 C57BL/6ByJ 小鼠的氯化钠消耗量和鼓索神经对舌侧氯化钠的反应。小鼠接受 300 mM NaCl（在 96 小时两瓶测试中饮用水）或注射醋酸脱氧皮质酮（DOCA；每天 33 mg/kg）。幼稚小鼠对 75 mM 氯化钠呈中性，但之前暴露于 300 mM 氯化钠的小鼠避免接触 75 mM 氯化钠。暴露于 NaCl（300 mM 4 天，75 mM 2 天）的小鼠鼓索中阿米洛利敏感成分对室温（24°C）下施加的 10-30 mM NaCl 的反应增强。 DOCA 注射增加了对 300 mM 氯化钠的接受度，但没有改变鼓索对 100-1000 mM 氯化钠的反应。然而，DOCA 治疗的小鼠鼓索中阿米洛利敏感成分对冷（12°C）10-30 mM NaCl 的反应增强。这些数据表明，外周味觉反应可能与暴露于浓缩氯化钠引起的氯化钠厌恶有关，但与 DOCA 诱导的氯化钠接受度增加无关。
To investigate the possible role of peripheral gustatory responsiveness to changes in NaCl acceptance, we studied NaCl consumption and the chorda tympani nerve responses to lingual application of NaCl in C57BL/6ByJ mice. The mice were treated with 300 mM NaCl (given to drink in 96-h two-bottle tests with water) or with injections of deoxycorticosterone acetate (DOCA; 33 mg/kg daily). Naı̈ve mice were neutral to 75 mM NaCl, but mice previously exposed to 300 mM NaCl avoided 75 mM NaCl. The NaCl-exposed (300 mM for 4 days and 75 mM for 2 days) mice had enhanced amiloride-sensitive components of the chorda tympani responses to 10–30 mM NaCl applied at room temperature (24°C). DOCA injections increased acceptance of 300 mM NaCl, but did not change the chorda tympani responses to 100–1000 mM NaCl. However, the DOCA-treated mice had enhanced amiloride-sensitive components of the chorda tympani responses to cold (12°C) 10–30 mM NaCl. These data suggest that peripheral gustatory responsiveness possibly contributes to the NaCl aversion induced by exposure to concentrated NaCl, but not to the DOCA-induced increase of NaCl acceptance.