A novel higher plant protein tyrosine phosphatase interacts with SNF1-related protein kinases via a KIS (kinase interaction sequence) domain
A novel higher plant protein tyrosine phosphatase interacts with SNF1-related protein kinases via a KIS (kinase interaction sequence) domain
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DOI：
10.1046/j.1365-313x.2002.01250.x
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发表时间：
2002-03-01
期刊：
影响因子：
7.2
通讯作者：
Gatehouse, JA
中科院分区：
文献类型：
--
作者：
Fordham-Skelton, AP;Chilley, P;Gatehouse, JA
通过数据库检索，在拟南芥中发现了一个新的蛋白磷酸酶。这种蛋白，命名为AtPTPKIS 1，含有蛋白酪氨酸磷酸酶（PTP）催化结构域和激酶相互作用序列（KIS）结构域。预计它会与植物SNF 1相关激酶（SnRKS）相互作用，这些激酶代表代谢和应激反应的中央调节因子。AtPTPKIS 1在双子叶植物和单子叶植物中有相似的同源物，但在其他植物界中没有发现。AtPTPKIS 1的番茄同源物表达为重组蛋白，并显示水解通用磷酸酶底物和合成肽中的磷酸酪氨酸残基。AtPTPKIS 1的KIS结构域被证明与植物SnRK AKIN 11在酵母双杂交系统中的体内相互作用，以及在GST融合“下拉”测定中的体外相互作用。拟南芥和其他植物的基因组包含进一步预测的蛋白质相关的AtPTPKIS 1，这也可以与SnRKs相互作用，并在新的调控和信号通路。
A novel protein phosphatase in Arabidopsis thaliana was identified by database searching. This protein, designated AtPTPKIS1, contains a protein tyrosine phosphatase (PTP) catalytic domain and a kinase interaction sequence (KIS) domain. It is predicted to interact with plant SNF1-related kinases (SnRKs), representing central regulators of metabolic and stress responses. AtPTPKIS1 has close homologues in other plant species, both dicots and monocots, but is not found in other kingdoms. The tomato homologue of AtPTPKIS1 was expressed as a recombinant protein and shown to hydrolyse a generic phosphatase substrate, and phosphotyrosine residues in synthetic peptides. The KIS domain of AtPTPKIS1 was shown to interact with the plant SnRK AKIN11 both in vivo in the yeast two-hybrid system, and in vitro in a GST-fusion 'pull down' assay. The genomes of Arabidopsis and other plants contain further predicted proteins related to AtPTPKIS1, which could also interact with SnRKs and act in novel regulatory and signalling pathways.