Effects of neonatal spleen cell products on the multiplication of toxoplasma in rat peritoneal macrophages.
Effects of neonatal spleen cell products on the multiplication of toxoplasma in rat peritoneal macrophages.
复制标题
新生儿脾细胞产物对大鼠腹腔巨噬细胞弓形虫增殖的影响。
DOI：
10.4049/jimmunol.131.3.1527
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发表时间：
1983
影响因子：
4.4
通讯作者：
N. Suzuki
中科院分区：
文献类型：
--
作者：
H. Sakurai;I. Igarashi;Y. Omata;A. Saito;N. Suzuki
我们研究了新生大鼠对弓形虫感染的耐药性。慢性感染大鼠的新生儿对弓形虫感染的抵抗力明显高于正常未感染大鼠的新生儿。免疫母鼠的新生胸大鼠（nu/nu）对感染易感，而正常窝鼠（nu/+）对感染具有很强的抵抗力。在培养基中加入免疫血清后，大鼠腹腔巨噬细胞单层未表现出增强的弓形虫杀灭活性。然而，在巨噬细胞感染前将弓形虫暴露于免疫血清中，观察到弓形虫增殖受到抑制。研究了慢性感染母鼠新生儿脾细胞产物对弓形虫在大鼠腹膜巨噬细胞中增殖的影响。巨噬细胞与免疫新生大鼠脾细胞培养的上清液孵育，并添加裂解抗原，可显著抑制弓形虫增殖。在出生后第三周，被诱导制造抑制上清的能力消失。还检测了脾细胞的上清液与弓形虫裂解物抗原孵育致敏；当这些抗原加入到巨噬细胞单层时，只有当抗原与免疫脾细胞上清一起时，才能观察到弓形虫增殖的抑制。
We studied the resistance of newborn rats to infection with T. gondii. Neonates of chronically infected rats were significantly more resistant to infection with Toxoplasma than were the newborn of normal, uninfected rats. Neonatal athymic rats (nu/nu) from immune mothers were susceptible to infection, whereas normal littermates (nu/+) were very resistant. Rat peritoneal macrophage monolayers did not exhibit enhanced toxoplasmacidal activity after the addition of immune serum to the culture medium. However, inhibition of Toxoplasma multiplication was observed when the parasites were exposed to immune serum just before the infection of macrophages. The effects of spleen cell products from neonates of chronically infected mothers on the multiplication of Toxoplasma in rat peritoneal macrophages were examined. A significant inhibition of Toxoplasma multiplication occurred in macrophages incubated with the supernatants of spleen cell cultures obtained from immune newborn rats, with added lysate antigen. The ability to be induced to make an inhibitory supernatant disappeared by the third week after birth. Also examined were supernatants of spleen cells sensitized in vitro by incubation with a Toxoplasma lysate antigen; when these were added to macrophage monolayers, inhibition of Toxoplasma multiplication was observed only if the antigen was accompanied by supernatant of immune spleen cells.