Sprouty, an intracellular inhibitor of Ras signaling
Sprouty, an intracellular inhibitor of Ras signaling
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DOI：
10.1016/s0092-8674(00)80576-0
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发表时间：
1999-03-05
期刊：
影响因子：
64.5
通讯作者：
Freeman, M
中科院分区：
文献类型：
--
作者：
Casci, T;Vinós, J;Freeman, M
Sprouty 在基因筛选中被鉴定为果蝇 EGF 受体信号传导的抑制剂。 Egfr 会触发眼睛中的细胞募集，而萌芽（-）的眼睛有多余的感光器、视锥细胞和色素细胞。然而，斯普劳蒂的功能更为广泛。我们发现它还与受体酪氨酸激酶 Torso 和 Sevenless 存在遗传相互作用，并且它是通过对 FGF 受体信号传导的影响而首次被发现的。与早期提出的 Sprouty 是细胞外蛋白相反，我们通过生化分析表明 Sprouty 是一种细胞内蛋白，与质膜的内表面相关。 Sprouty 与 Ras 通路的两个细胞内成分 Drk 和 Gap1 结合。我们的结果表明 Sprouty 是 Ras 通路信号转导的广泛抑制剂。
Sprouty was identified in a genetic screen as an inhibitor of Drosophila EGF receptor signaling. The Egfr triggers cell recruitment in the eye, and sprouty(-) eyes have excess photoreceptors, cone cells, and pigment cells. Sprouty's function is, however, more widespread. We show that it also interacts genetically with the receptor tyrosine kinases Torso and Sevenless, and it was first discovered through its effect on FGF receptor signaling. In contrast to an earlier proposal that Sprouty is extracellular, we show by biochemical analysis that Sprouty is an intracellular protein, associated with the inner surface of the plasma membrane. Sprouty binds to two intracellular components of the Ras pathway, Drk and Gap1. Our results indicate that Sprouty is a widespread inhibitor of Ras pathway signal transduction.