Rapid control of protein level in the apicomplexan Toxoplasma gondii
Rapid control of protein level in the apicomplexan Toxoplasma gondii
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DOI：
10.1038/nmeth1134
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发表时间：
2007-12-01
期刊：
影响因子：
48
通讯作者：
Meissner, Markus
中科院分区：
文献类型：
--
作者：
Herm-Goetz, Angelika;Agop-Nersesian, Carolina;Meissner, Markus
由于缺乏能够轻松快速调节蛋白质水平的反向遗传工具，对顶复门寄生虫的基因功能分析受到限制。配体控制的去稳定结构域 (ddFKBP) 与目标蛋白质的融合能够在弓形虫中实现快速、可逆的蛋白质稳定。这使得能够对在宿主细胞入侵和/或寄生虫细胞内生长期间具有双重作用的蛋白质进行有效的功能分析。
Analysis of gene function in apicomplexan parasites is limited by the absence of reverse genetic tools that allow easy and rapid modulation of protein levels. The fusion of a ligand-controlled destabilization domain (ddFKBP) to a protein of interest enables rapid and reversible protein stabilization in T. gondii. This allows an efficient functional analysis of proteins that have a dual role during host cell invasion and/or intracellular growth of the parasite.