Regulation of Poly(A) Tail and Translation during the Somatic Cell Cycle
Regulation of Poly(A) Tail and Translation during the Somatic Cell Cycle
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DOI：
10.1016/j.molcel.2016.04.007
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发表时间：
2016-05-05
期刊：
影响因子：
16
通讯作者：
Kim, V. Narry
中科院分区：
文献类型：
--
作者：
Park, Jong-Eun;Yi, Hyerim;Kim, V. Narry
Poly（A）尾对mRNA的稳定性和翻译至关重要。然而，最近的研究挑战了这一观点，表明poly（A）尾长和翻译效率在非胚胎细胞中是解耦的。使用TAIL-seq和核糖体分析，我们研究了体细胞周期中的poly（A）尾动力学和翻译控制。我们发现细胞周期调控基因如CDK 1，TOP 2A和FBXO 5的poly（A）尾长度发生了显着变化，解释了它们在M期的翻译抑制。我们还发现当poly（A）尾为20个核苷酸poly（A）尾时，poly（A）尾的长度与翻译相关，在细胞周期中，poly（A）尾的翻译主要通过poly（A）尾长度非依赖性机制来调节。具体来说，我们发现，终端寡嘧啶（TOP）trac-containing转录逃脱全球翻译抑制M期，积极翻译。我们的定量和全面的数据提供了一个修改后的体细胞周期中的翻译控制的观点。
Poly(A) tails are critical for mRNA stability and translation. However, recent studies have challenged this view, showing that poly(A) tail length and translation efficiency are decoupled in non-embryonic cells. Using TAIL-seq and ribosome profiling, we investigate poly(A) tail dynamics and translational control in the somatic cell cycle. We find dramatic changes in poly(A) tail lengths of cell-cycle regulatory genes like CDK1, TOP2A, and FBXO5, explaining their translational repression in M phase. We also find that poly(A) tail length is coupled to translation when the poly(A) tail is 20 nucleotide poly(A) tails, their translation is regulated mainly via poly(A) tail length-independent mechanisms during the cell cycle. Specifically, we find that terminal oligopyrimidine (TOP) tract-containing transcripts escape global translational suppression in M phase and are actively translated. Our quantitative and comprehensive data provide a revised view of translational control in the somatic cell cycle.