Invasion assays.
Invasion assays.
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DOI：
10.1002/0471143030.cb1202s00
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发表时间：
2001-05-01
影响因子：
--
通讯作者：
Jacob, K
中科院分区：
文献类型：
--
作者：
Kleinman, H K;Jacob, K
基底膜是位于上皮细胞和内皮细胞之下并将它们与基质分离的薄细胞外基质。肿瘤细胞必须穿过这层膜侵入间质并建立远处转移。它们通过产生降解基质的蛋白酶来实现这一点。在几种体外模型中，使用Matrigel的模型是最可靠、可重复和代表体内侵袭的模型。在本文所示的测定中，将肿瘤细胞置于Boyden迁移室的上室中。上腔室和下腔室由涂覆有Matrigel的多孔膜隔开。下孔中的化学引诱物刺激迁移。间隔一段时间后，从膜的下表面回收肿瘤细胞或在膜的下表面上计数肿瘤细胞。
Basement membrane is a thin extracellular matrix that underlies epithelia and endothelia and separates them from the stroma. Tumor cells must cross this membrane to invade stroma and establish distant metastases. They do this by producing proteases that degrade the matrix. Of several in vitro models, those using Matrigel are the most reliable, reproducible, and representative of in vivo invasion. In the assay presented here, tumor cells are placed in the upper chamber of Boyden migration chamber. The upper and lower chambers are separated by a porous membrane coated with Matrigel. A chemoattractant in the lower well stimulates migration. After an interval, tumor cells are recovered from or counted on the lower surface of the membrane.