Both post-Golgi and intra-Golgi cycling affect the distribution of the Golgi phosphoprotein GPP130.
Both post-Golgi and intra-Golgi cycling affect the distribution of the Golgi phosphoprotein GPP130.
复制标题
高尔基体后和高尔基体内循环都会影响高尔基体磷蛋白 GPP130 的分布。
DOI：
10.1111/j.1600-0854.2007.00607.x
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Storrie,Brian
中科院分区：
文献类型：
--
作者：
Starr,Tregei;Forsten-Williams,Kimberly;Storrie,Brian
高尔基体磷蛋白、GPP130、acisGolgi 蛋白是以 pH 敏感方式在高尔基体和内体之间循环的蛋白质的代表。目前的定性数据不足以区分高尔基体和内体过程在调节此类蛋白质循环中的相对贡献。我们采用定量方法来分析 GPP130 对 pH 扰动的分布。我们使用志贺样毒素 B 片段（一种通过晚期内体旁路途径从细胞表面和高尔基体运输的蛋白质）作为探针来突出 GPP130 循环的一个方面，并且类似地使用 tsO45-绿色荧光蛋白（GFP）在高尔基体和质膜之间的运输来单独处理 GPP130 循环的这一方面。总的来说，我们从定量分析和模拟中得出结论，用 pH 扰动物莫能菌素处理 HeLa 细胞会在几个阶段影响 GPP130 循环，对 (i) 高尔基体内循环、(ii) 反式高尔基体到内体的转运和 (iii) 内体到高尔基体的转运产生影响。我们的分析表明，反式高尔基体（高尔基体中酸性最强的部分）的影响最大。总之，多个受监管的步骤影响 GPP130 的贩运。
Golgi phosphoprotein, GPP130, acisGolgi protein, is representative of proteins cycling between the Golgi apparatus and endosomes in a pH‐sensitive manner. The present qualitative data are insufficient to distinguish the relative contributions of Golgi and endosomal processes in regulating the cycling of such proteins. We have taken a quantitative approach to analyze GPP130 distribution in response to pH perturbation. We have used Shiga‐like toxin B fragment, a protein that traffics from the cell surface and Golgi apparatus by the late endosomal bypass pathway, as a probe to highlight one aspect of GPP130 cycling and similarly the trafficking of tsO45‐green fluorescent protein (GFP) between the Golgi apparatus and the plasma membrane to treat that aspect of GPP130 cycling in isolation. Overall, we conclude from quantitative analysis and simulations that treatment of HeLa cells with the pH perturbant, monensin, affects GPP130 cycling at several stages with effects on (i) intra‐Golgi cycling, (ii)transGolgi to endosome transport and (iii) endosome to Golgi transport. Our analysis indicates that the effect is greatest at thetransGolgi, the most acidic portion of the Golgi apparatus. In sum, multiple, regulated steps affect the trafficking of GPP130.