Human herpesvirus (HHV)-6 and HHV-7 infections in pregnant women
Human herpesvirus (HHV)-6 and HHV-7 infections in pregnant women
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DOI：
10.1086/522430
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发表时间：
2007-11-01
影响因子：
6.4
通讯作者：
McDermott, Michael P.
中科院分区：
文献类型：
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作者：
Caserta, Mary T.;Hall, Caroline Breese;McDermott, Michael P.
背景资料。人类疱疹病毒(HHV)-6和HHV-7的宫内和性传播已被认为是可能的，但也存在先天性HHV-6感染。我们前瞻性地研究了孕妇体内多个地点的HHV-6和HHV-7，以确定这些病毒在重复时间点的特征。用套式聚合酶链式反应和逆转录聚合酶链式反应检测外周血单个核细胞、宫颈分泌物、胎盘和脐带血中的HHV-6和HHV-7，并用定量聚合酶链式反应检测HHV-6。同时对一组女性进行了研究。我们招募了104名孕妇和31名对照妇女。孕妇外周血中HHV-7DNA的阳性率(66.9%)高于HHV-6DNA的阳性率(22.2%；P=0.19)，而宫颈拭子中HHV-7DNA和HHV-6DNA的阳性率均较低(3.0%对7.5%；P=0.0001)。宫颈拭子中存在HHV-6DNA的孕妇与宫颈拭子阴性的孕妇相比，血液中存在HHV-6DNA的几率更高(优势比为12.9；P=.0009)。17%的孕妇建议HHV-6重新激活或再次感染。1例胎盘标本中有HHV-6复制活性。宫颈分泌物中HHV-6DNA的检测与PBMC中HHV-6DNA的检测具有相关性。活动性胎盘感染与先天性HHV-6感染一起被确认。
Background. Both intrauterine and sexual transmission of human herpesvirus (HHV)-6 and HHV-7 have been suggested, and congenital HHV-6 infection does occur. We prospectively studied HHV-6 and HHV-7 at multiple sites in pregnant women to determine the characteristics of these viruses at repeated time points.Methods. Peripheral blood mononuclear cells (PBMCs), cervical secretions, placenta, and cord blood were tested by nested polymerase chain reaction (PCR) and reverse-transcriptase PCR for HHV-6 and HHV-7 and by quantitative PCR for HHV-6. A control group of women was also studied.Results. We enrolled 104 pregnant and 31 control women. HHV-7 DNA was detected more frequently in PBMCs from pregnant women (66.9%) than HHV-6 DNA (22.2%; P = .19), but both were found at low rates in cervical swabs (HHV-7 vs. HHV-6 DNA, 3.0% vs. 7.5%; P = .0001). Pregnant women with HHV-6 DNA present in cervical swabs had a greater odds of having HHV-6 DNA present in the blood than did pregnant women with negative cervical swabs (odds ratio, 12.9; P = .0009). HHV-6 reactivation or reinfection was suggested in 17% of pregnant women. One placental sample had active HHV-6 replication.Conclusions. Detection of HHV-6 DNA in cervical secretions is associated with HHV-6 DNA in PBMC samples. Active placental infection along with congenital HHV-6 infection was identified.