Isolation and characterization of a novel single-stranded RNA virus infecting the bloom-forming diatom Rhizosolenia setigera
Isolation and characterization of a novel single-stranded RNA virus infecting the bloom-forming diatom Rhizosolenia setigera
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DOI：
10.1128/aem.70.2.704-711.2004
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发表时间：
2004-02-01
影响因子：
4.4
通讯作者：
Yamaguchi, M
中科院分区：
文献类型：
--
作者：
Nagasaki, K;Tomaru, Y;Yamaguchi, M
从日本有里亚海分离到一种新的单链RNA病毒(单链RNA病毒)，该病毒特异性地侵染硅藻根霉(Rhizosolenia Setigera)[RsRNAV]。病毒复制发生在细胞质内，病毒颗粒呈二十面体，无尾巴，平均直径32 nm。从RsRNAV颗粒中提取的主要核酸是11.2kb的单链RNA分子，但偶尔也会观察到较小的RNA分子(0.6、1.2和1.5kb)。RsRNAV的主要结构蛋白为41.5、41.0和29.5 kDa。RsRNAV的种间和种内宿主特异性试验表明，RsRNAV不仅具有种属特异性，而且具有株系特异性，其种内宿主特异性在不同的病毒克隆中是不同的。RsRNAV的潜伏期为2天，病毒在对数生长期和稳定期分别在15℃和12小时-12小时的光暗周期下接种到寄主细胞中，爆发大小分别为3,100和1,010个病毒/宿主细胞，光照为冷白光，光强为110×10~(-2)×10~(-1)。据我们所知，这是第一份描述感染硅藻的病毒的生物学特性的报告。对RsRNAV的进一步研究将有助于理解自然界中硅藻和病毒之间的生态关系。
A novel single-stranded RNA (ssRNA) virus specifically infecting the bloom-forming diatom Rhizosolenia setigera (R. setigera RNA virus [RsRNAV]) was isolated from Ariake Sea, Japan. Viral replication occurred within the cytoplasm, and the virus particle was icosahedral, lacked a tail, and was 32 nm in diameter on average. The major nucleic acid extracted from the RsRNAV particles was an ssRNA molecule 11.2 kb in length, although smaller RNA molecules (0.6, 1.2, and 1.5 kb) were occasionally observed. The major structural proteins of RsRNAV were 41.5, 41.0, and 29.5 kDa. Inter- and intraspecies host specificity tests revealed that RsRNAV is not only species specific but also strain specific and that its intraspecies host specificity is diverse among virus clones. The latent period of RsRNAV was 2 days, and the burst sizes were 3,100 and 1,010 viruses per host cell when viruses were inoculated into the host culture at the exponential and stationary growth phases, respectively, at 15degreesC under a 12-h-12-h light-dark cycle of ca. 110 mumol of photons m(-2) s(-1) with cool white fluorescent illumination. To our knowledge, this is the first report describing the biological properties of a virus infecting a diatom. Further studies on RsRNAV will be helpful in understanding the ecological relationship between diatoms and viruses in nature.