Surfactant proteins A and D in the genital tract of mares
Surfactant proteins A and D in the genital tract of mares
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DOI：
10.1016/j.anireprosci.2006.03.009
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发表时间：
2007-04-01
影响因子：
2.2
通讯作者：
Gungor, Orsan
中科院分区：
文献类型：
--
作者：
Kankavi, Orhan;Ata, Ayhan;Gungor, Orsan
几十年来，肺泡中表面活性物质的存在被认为是正常肺功能所必需的。表面活性剂对于降低肺泡气液界面的表面张力是必不可少的。肺表面活性物质由90%脂质和10%蛋白质组成。表面活性蛋白A（SP-A）、表面活性蛋白B（SP-B）、表面活性蛋白C（SP-C）和表面活性蛋白D（SP-D）是四种非血清蛋白。肺SPA和SP-D属于一个含有胶原的C型凝集素家族，称为collectins。SP-A和SP-D最初在肺泡II型细胞中被证实，但最近的研究表明SP-A和SP-D的肺外表达表明这些蛋白质的全身作用。本研究描述了SP-A和SP-D的存在下，母马生殖道，外阴，阴道，卵巢，子宫和输卵管uterina使用免疫组化和Western blotting.本研究的目的是表征表面活性蛋白的条款：（i）表面活性蛋白是否存在于母马生殖系统的各种结构中，（ii）如果存在，识别和定位表面活性蛋白，以及最后（iii）确定与先前表征的肺表面活性蛋白的差异。虽然超出了本报告的范围，但人们认识到，更好地定义母马的生殖防御机制也有一些潜在的影响。因此，检查了生殖系统器官和组织。我们能够表明，与表面活性剂特异性抗体反应的蛋白质存在于母马生殖道。因此，表面活性蛋白不仅存在于与肺相关的板层体中，而且存在于母马的生殖系统中。(c)2006 Elsevier B. V.保留所有权利。
The presence of surface-active material in the lung alveolus has been known for several decades as being essential for normal lung function. Surfactant is essential for reducing the surface tension at the alveolar air-liquid interface. Pulmonary surfactant is composed of 90% lipids and 10% proteins. There are four non-serum proteins surfactant protein-A (SP-A), surfactant protein-B (SP-B), surfactant protein-C (SP-C) and surfactant protein-D (SP-D) named in chronologic, order of discovery. Lung SPA and SP-D belong to a family of collagen-containing C-type lectin family called collectins. The host defence and controlling inflammatory processes of the lung are the major functions of SP-A and SP-D.SP-A and SP-D were originally demonsrated in alveolar type II cells, but recent studies have shown extrapulmonary expression of SP-A and SP-D indicating systemic roles of these proteins. Present study decribes the presence of SP-A and SP-D in the mare genital tract, vulva, vagina, ovarium, uterus and tuba uterina using immunohistochemistry and Western blotting.The aim of this study was to characterize surfactant proteins in terms of: (i) whether surfactant proteins were present in the various structures of the mare genital system, (ii) if so, identifying and locating the surfactant proteins and finally (iii) determining the differences from those previously characterized for the lung. Although beyond the scope of this report, it is recognized that there are also some potential implications for better defining the reproductive defence mechanisms in mare. Therefore, genital system organs and tissues from mares were examined. We were able to show that proteins reactive with surfactant-specific antibodies were present in the mare genital tract. Thus, surfactant proteins are present not in just lamellar bodies associated with lung, but also genital system of mare. (c) 2006 Elsevier B.V. All rights reserved.