Genetic Diversity and Allelic Frequency of Glutamate-Rich Protein (GLURP) in Plasmodium falciparum Isolates from Sub-Saharan Africa.
Genetic Diversity and Allelic Frequency of Glutamate-Rich Protein (GLURP) in Plasmodium falciparum Isolates from Sub-Saharan Africa.
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DOI：
10.4137/mbi.s20618
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发表时间：
2014-01-01
影响因子：
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通讯作者：
Thomas, Bolaji N
中科院分区：
文献类型：
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作者：
Duru, Kimberley C;Thomas, Bolaji N
富含谷氨酸的蛋白是恶性疟原虫（Pf）的抗原，存在于疟原虫的所有阶段，并已报道可诱导临床免疫。已经发现R0和R2区域表现出高度的保守性，因此用作良好的疫苗设计材料。我们分析了Pf glurp基因在R0和R2区域的遗传多样性，并评估了季节性对等位基因频率的作用。共402个基因组DNA样本，从滤纸血样本中提取，筛选通过巢式聚合酶链反应（PCR）分析Pf glurp R0和R2区域，除了多态性区域的片段分析，以确定等位基因多样性的寄生虫种群。我们发现了广泛的异质性在R2区域一般，这种异质性是季节性依赖，区域可塑性的指示。R0区域显示出遗传保守性，正如预期的那样。我们的结论是，积极的基因分型结果与glurp R0区域应被视为一个积极的Pf感染的指示，需要适当的治疗。此外，我们主张扩大R0区基因型阳性可作为诊断工具的可能性，从而减少未经治疗或治疗不良的感染病例，从而有助于复发或治疗失败。
Glutamate-rich protein is a Plasmodium falciparum (Pf) antigen found in all stages of the parasite and has been reported to induce clinical immunity. The R0 and R2 regions have been found to exhibit a high degree of conservation, therefore serving as a good vaccine design material. We assayed the genetic diversity of Pf glurp genes in the R0 and R2 regions, as well as evaluated the role of seasonality on allelic frequency. A total of 402 genomic DNA samples, extracted from filter paper blood samples, were screened by nested polymerase chain reaction (PCR) analysis of Pf glurp R0 and R2 regions, in addition to fragment analysis of the polymorphic regions to identify allelic diversity of the parasite population. We found an extensive heterogeneity in the R2 region in general, and this heterogeneity is seasonally dependent, indicative of region plasticity. The R0 region displayed genetic conservation, as expected. We conclude that positive genotyping results with glurp R0 region should be seen as indicative of an active Pf infection, requiring adequate treatment. In addition, we advocate extending the possibility that an R0 region genotypic positivity could serve as diagnostic tool, thereby reducing cases of untreated or poorly treated infection, contributory to recrudescence or treatment failure.