Evaluation of silver nanoparticle toxicity to RAW264.7 cells in a three-dimensional cell culture.
Evaluation of silver nanoparticle toxicity to RAW264.7 cells in a three-dimensional cell culture.
复制标题
三维细胞培养物中银纳米颗粒对 RAW264.7 细胞的毒性评估。
DOI：
10.1166/jbt.2014.1141
复制
发表时间：
2014
影响因子：
0.1
通讯作者：
Endo K
中科院分区：
文献类型：
--
作者：
Hashimoto M;Toshima H;Yonezawa T;Kawai K;Iijima M;Endo K
需要临床相关的体外模型来快速评估纳米颗粒的细胞毒性。最近，已经制造了许多三维细胞培养系统（3D）以模拟体内组织的性质。球体培养模型在理解肿瘤生物学和进行药物和生物材料筛选方面发挥着不可估量的作用。纳米颗粒（NPs）目前在工业和生物医学应用中受到相当大的关注，但其危险性和潜在的毒性作用尚未得到广泛研究。本研究的目的是评价聚乙烯吡咯烷酮包被的银纳米颗粒（AgNPs）在传统二维（2D）和新型三维系统中培养的小鼠巨噬细胞（RAW 264. 7细胞）中的生物学效应。进行生物学测定和微形态学分析。显微成像显示，颗粒被内化到细胞中，并发生受体介导的内吞作用。然而，与未暴露的细胞相比，暴露于AgNPs的细胞表现出轻微的形态学变化。AgNPs在2D培养中显示出比在3D中更大的细胞毒性。此外，在3D培养中，AgNP细胞毒性随时间降低，这在2D培养中没有发生。
Clinically relevant in vitro models are required to rapidly evaluate the cytotoxicity of nanoparticles. Recently, numerous three-dimensional cell culture systems (3D) have been manufactured to mimic the properties of tissue in vivo. Spheroid culture models play an invaluable role in understanding tumor biology and in performing the screening of drugs and biomaterials. Nanoparticles (NPs) are currently receiving considerable attention for industrial and biomedical applications, yet their hazardous and potential toxic effects have not been studied extensively. The purpose of this study was to evaluate the biological effects of polyvinylpyrrolidone-coated silver nanoparticles (AgNPs) in murine macrophages (RAW264.7 cells) cultured in traditional two-dimensional (2D) and novel 3D systems. Biological assays and a micromorphological analysis was performed. Microscopic imaging revealed that the particles were internalized into cells and receptor-mediated endocytosis occurred. However, cells exposed to AgNPs exhibited minor morphological changes compared with cells no exposed. AgNPs displayed greater cytotoxicity in the 2D culture versus in the 3D. Moreover, in the 3D culture the AgNP cytotoxicity decreased over time, which did not occur in the 2D culture.