Multigenerational effects of 4-methylbenzylidene camphor (4-MBC) on the survival, development and reproduction of the marine copepod Tigriopus japonicus
Multigenerational effects of 4-methylbenzylidene camphor (4-MBC) on the survival, development and reproduction of the marine copepod Tigriopus japonicus
复制标题
4-甲基亚苄基樟脑（4-MBC）对海洋桡足类虎鱼生存、发育和繁殖的多代效应
DOI：
10.1016/j.aquatox.2017.11.008
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发表时间：
2018-01-01
影响因子：
4.5
通讯作者：
Shi, Dalin
中科院分区：
文献类型：
--
作者：
Chen, Leyun;Li, Xiaolin;Shi, Dalin
最广泛使用的有机紫外线过滤器之一，4-甲基苄基樟脑（4-MBC），在近海水域中浓度很高。以日本虎足（Tigriopus japonicus）为研究对象，连续4代（f0 ~ f3）暴露于不同浓度的4- mbc(0、0.5、1、5、10 μ g -1)，评价4- mbc对海洋生态系统的影响。结果表明，4-MBC在5和10 μ g L-1浓度下对日本赤霉病螨具有显著的致死毒性，赤霉病螨对4-MBC的毒性反应比成虫更敏感。而在f1 ~ f3代中，4-MBC暴露不影响成活率。在最低暴露浓度（0.5 μ g L-1）下，F1-F2代和F2-F3代的小鳞蝽到桡足虫（N-C）和成虫（N-A）的孵化率和发育时间均显著降低。在随后的两代（即F4-F5代）清洁海水恢复暴露中，在N-C和N-A阶段，原始4-MBC暴露组的生长速度仍然快于对照，表明慢性4-MBC暴露可能发生了跨代遗传和/或表观遗传变化。4-MBC上调了蜕皮激素受体基因的表达，这与N-C/N-A持续时间的降低一致。此外，4-MBC还可能诱导日本刺参氧化应激并引发细胞凋亡，从而导致发育、生殖甚至致死毒性。初步风险评估表明，在符合环境实际的浓度下，4-MBC对海洋甲壳类动物和海洋生态系统具有显著的潜在威胁。
One of the most widely used organic UV filters, 4-methylbenzylidene camphor (4-MBC), is present at high concentrations in offshore waters. The marine copepod Tigriopus japonicus was exposed to different concentrations of 4-MBC (i.e., 0, 0.5, 1, 5 and 10 mu g L-1) for 4 consecutive generations (F0-F3) to evaluate the impact of 4-MBC on marine ecosystems. The results showed that in the FO generation, 4-MBC caused significant lethal toxicity in T. japonicas at concentrations of 5 and 10 mu g L-1 and the nauplii were more sensitive to 4-MBC toxicity than the adults. However in the F1-F3 generations, 4-MBC exposure did not affect the survival rate. The hatching rate and the developmental duration from the nauplii to the copepodite (N-C) and from the nauplii to adult (N-A) decreased significantly in the F1-F2 generations and in the F2-F3 generations, respectively, even at the lowest exposure concentration (0.5 mu g L-1). In the subsequent two generations (i.e., the F4-F5 generations) of recovery exposure in clean seawater, the growth rates of the original 4-MBC exposure groups were still faster than the control in both the N-C and N-A stages, suggesting possible transgenerational genetic and/or epigenetic changes upon chronic 4-MBC exposure. The expression of the ecdysone receptor gene was up-regulated by 4-MBC, which was consistent with the decrease of the N-C/N-A duration. In addition, 4-MBC may induce oxidative stress and trigger apoptosis in T. japonicas, resulting in developmental, reproductive and even lethal toxicity. A preliminary risk assessment suggested that under environmentally realistic concentrations, 4-MBC had significant potential to pose a threat to marine crustaceans and marine ecosystems.