Automatic Time-Resolved Fluorescence Immunoassay of Serum Alpha Fetoprotein-L3 Variant via LCA Magnetic Cationic Polymeric Liposomes Improves the Diagnostic Accuracy of Liver Cancer
Automatic Time-Resolved Fluorescence Immunoassay of Serum Alpha Fetoprotein-L3 Variant via LCA Magnetic Cationic Polymeric Liposomes Improves the Diagnostic Accuracy of Liver Cancer
复制标题
通过 LCA 磁性阳离子聚合物脂质体对血清甲胎蛋白-L3 变体进行自动时间分辨荧光免疫分析可提高肝癌的诊断准确性
DOI：
10.2147/ijn.s242527
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发表时间：
2020-01-01
影响因子：
8
通讯作者：
Liang, Xiaofei
中科院分区：
文献类型：
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作者：
Wang, Kai;Li, Yuzhong;Liang, Xiaofei
本研究以扁豆凝集素衍生物（LCA-HQ）和胆固醇为原料，采用薄膜水化法制备了凝集素修饰的大分子脂质磁性微球，并对其在肝病和肝癌鉴别诊断中的应用进行了研究。用制备的凝集素修饰的高分子脂质磁性微球分离健康人、肝病患者和肝癌患者血清中的甲胎蛋白变体（AFP-L3），并用全自动时间分辨荧光免疫分析法检测甲胎蛋白（AFP）和AFP-L3。亲和素磁珠-全自动时间分辨荧光免疫分析法与手工微量亲和柱法检测AFP-L3的回收率差异无统计学意义（p>0.05）。我们发现AFP-L3可以作为肝癌和肝病之间的鉴别指标。肝癌患者AFP和AFP-L3阳性率明显高于健康人和肝病患者（P<0.001）。AFP和AFP-L3的AUC（95% CI）分别为0.743 ± 0.031和0.850 ± 0.024。AFP-L3的AUC值大于AFP，因此AFP-L3区分肝癌更准确，差异有统计学意义，p<0.05。结论我们提出了一种将凝集素聚合物脂质磁性球与时间分辨荧光免疫分析相结合的新方法，可以简单、准确、快速地测定临床样本中的AFP-L3。值得注意的是，全自动时间分辨荧光免疫分析与临床上常用的技术相比，测量程序简单，有望用于肝癌的检测和准确诊断。
PurposeThe aim of this study was to develop an avidin-modified macromolecular lipid magnetic sphere and its application in differential diagnosis of liver disease and liver cancer.Materials and MethodsLectin-modified macromolecular lipid magnetic spheres were prepared by thin-film hydration method using lentil lectin derivatives (LCA-HQ) and cholesterol as raw materials. Alpha-fetoprotein variants (AFP-L3) in serum from healthy people, liver disease and liver cancer patients were isolated using the prepared lectin-modified macromolecular lipid magnetic spheres, and alpha-fetoprotein (AFP) and AFP-L3 were detected by fully automatic time-resolved fluorescence immunoassay.ResultsThe lectin polymer lipid magnetic sphere prepared in this study was superparamagnetic and encapsulated by a lectin derivative. There was no significant difference in the recovery rate of AFP-L3 between avidin magnetic ball-automatic time-resolved fluorescence immunoassay and manual micro-affinity column method (p>0.05). We found that AFP-L3 can be used as a differential indicator between liver cancer and liver disease. The positive rate of AFP and AFP-L3 in liver cancer patients was higher than that in healthy people and liver disease patients (p<0.001). The AUC (95% CI) of AFP and AFP-L3 were 0.743 ± 0.031 and 0.850 ± 0.024, respectively. AFP-L3 AUC value is greater than AFP; therefore, AFP-L3 distinguishes liver cancer more accurately, and the difference is statistically different, p<0.05.ConclusionWe proposed a novel method for integration of the lectin polymer lipid magnetic spheres and time-resolved fluorescence immunoassay that enables simple, accurate and rapid determination of AFP-L3 in clinical samples. To be noted, fully automatic time-resolved fluorescence immunoassay compared with the commonly used techniques in clinical practice, the measurement procedure is simple and is expected to be used for the detection and accurate diagnosis of liver cancer.