Identification of taste-tissue-specific cDNA clones from a subtraction cDNA library of rat circumvallate and foliate papillae
Identification of taste-tissue-specific cDNA clones from a subtraction cDNA library of rat circumvallate and foliate papillae
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DOI：
10.1093/oxfordjournals.jbchem.a022209
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发表时间：
1998-11-01
影响因子：
2.7
通讯作者：
Emori, Y
中科院分区：
文献类型：
--
作者：
Asano-Miyoshi, M;Kusakabe, Y;Emori, Y
为了鉴定味觉组织中特异表达的基因，我们构建了大鼠周乳头上皮和叶乳头上皮的消减cDNA文库，并对该文库进行了差异筛选，斑点印迹分析显示，该方法获得的88个克隆中有46个在乳头上皮中表达，对这些克隆中的cDNA插入片段进行了测序，并分析了与GenBank数据库条目的相似性。约54.3%的克隆是已知序列，包括艾伯纳林、细胞角蛋白18和Na+,K+-ATP酶的序列，原位杂交显示这些序列在周乳头中表达。大约 41.3% 的乳头特异性克隆与已知序列没有显着相似性，是新型味蕾特异性标记基因的候选者。
To identify genes specifically expressed in taste tissues, we constructed a subtraction cDNA library of epithelium of rat circumvallate and foliate papillae and carried out differential screening of this library, Dot blot analysis showed 46 out of 88 clones obtained by this method to be expressed in the epithelium of papillae, The cDNA inserts in these clones were sequenced and analyzed for similarity to entries the GenBank database. About 54.3% of the clones were known sequences, including the sequences of ebnerin, cytokeratin 18, and Na+,K+-ATPase, that were shown by in situ hybridization to be expressed in the circumvallate papillae. About 41.3% of the papillae-specific clones had no significant similarity to known sequences and are candidates for novel taste bud-specific marker genes.