FUNCTIONAL COMPARTMENTALIZATION OF OXIDATIVE AND GLYCOLYTIC METABOLISM IN VASCULAR SMOOTH-MUSCLE
FUNCTIONAL COMPARTMENTALIZATION OF OXIDATIVE AND GLYCOLYTIC METABOLISM IN VASCULAR SMOOTH-MUSCLE
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DOI：
10.1152/ajpcell.1983.244.5.c399
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发表时间：
1983-01-01
影响因子：
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通讯作者：
PAUL, RJ
中科院分区：
文献类型：
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作者：
PAUL, RJ
血管平滑肌的代谢以一种不寻常的有氧糖酵解成分为特征。即使在完全充氧的条件下，乳酸盐的产量也与氧气消耗的摩尔比率相似。尽管其潜在的机制尚不清楚，但糖酵解/氧化代谢的比率被认为是血管肌病的一个指标。测定了猪冠状动脉耗氧速率(JO2)、乳酸生成量(Jlac)和等长力(Jlac)，并在已知改变主动力(ΔPo)和Na+-K+转运的条件下进行了测定。正如先前报道的那样，JO2与ΔPo密切相关；然而，Jlac与改变Na+-K+转运的条件相关。在已知抑制Na~+-K~+转运的条件下(10~(-5)M哇巴因，无细胞外K~+或Na~+)，Jlac被抑制，即使ΔPo和JO_2增加。Na+-K+转运与有氧糖酵解的耦合不依赖于紧张度或主要阴离子种类，也不是简单地影响组织乳酸含量所表明的组织乳酸通透性。在类似浓度下进行的代谢测量表明，哇巴因对Jlac有明显的抑制作用，而JO2几乎没有影响。因此，在血管平滑肌中观察到的异常有氧糖酵解似乎与Na+-K+运输过程有关，而与某些非特异性代谢缺陷无关。对大鼠、兔、狗和猪的体动脉和肺动脉的实验表明，这些结果并不局限于猪的冠状动脉。
The metabolism of vascular smooth muscle is characterized by an unusual component of aerobic glycolysis. Lactate production, even under fully oxygenated conditions, is of similar magnitude to the rate of O2 consumption when compared on a molar basis. Although the underlying mechanisms are unknown, the ratio of glycolytic to oxidative metabolism was suggested to be an index of vascular myopathy. Measurements of the rate of O2 consumption (JO2), lactate production (Jlac) and isometric force in porcine coronary arteries were made under conditions known to alter both active force (.DELTA.Po) and Na+-K+ transport. As previously reported, JO2 was strongly correlated with .DELTA.Po; Jlac, however, was correlated with conditions that alter Na+-K+ transport. Under conditions known to inhibit Na+-K+ transport (10-5 M ouabain, absence of extracellular K+ or Na+), Jlac was inhibited even though .DELTA.Po and JO2 were increased. The coupling of Na+-K+ transport with aerobic glycolysis was not dependent on tonicity or the major anion species, nor was it an effect simply on tissue lactate permeability as indicated by studies of tissue lactate content. Metabolic measurements made at similar levels of .DELTA.Po indicate that Jlac is markedly inhibited by ouabain, whereas JO2 shows little effect. Thus the unusual aerobic glycolysis observed in vascular smooth muscle appears to be linked to Na+-K+ transport processes and not to some nonspecific metabolic deficiency. Experiments on both systemic and pulmonary arteries from rat, rabbit, dog and pig indicate that these results are not limited solely to porcine coronary vessels.