Recovery of ventricular function in reperfused ischemic rat hearts exposed to fatty acids.
Recovery of ventricular function in reperfused ischemic rat hearts exposed to fatty acids.
复制标题
暴露于脂肪酸的再灌注缺血大鼠心脏的心室功能恢复。
DOI：
10.1152/ajpheart.1985.249.3.h492
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Neely,JR
中科院分区：
文献类型：
--
作者：
Ichihara,K;Neely,JR
本文研究了缺血和再灌注心脏脂肪酸代谢产物的组织水平与再灌注心脏机械功能恢复的关系。离体大鼠心脏暴露于全球缺血的时间长达60分钟的各种条件下的冠状动脉流量，O2供应，和脂肪酸浓度，然后再灌注15或30分钟有氧条件下，有和没有脂肪酸存在。组织水平的ATP，磷酸肌酸，长链酰基辅酶A，长链酰基肉毒碱在缺血和再灌注期结束时进行了测定。在一些实验中，在缺血期间的K+停滞被用于防止在不存在和存在高脂肪酸的情况下腺嘌呤核苷酸耗尽。虽然这些心脏的能力，以恢复其缺血前的机械功能变化从8至90%和组织水平的酰基辅酶A和酰基肉毒碱在缺血期间变化从3至10倍取决于条件，没有相关性被发现之间的恢复功能在再灌注期间和存在的脂肪酸或高水平的组织长链酰基辅酶A和肉毒碱酯在缺血期间。
The relationship between tissue levels of fatty acid metabolites in ischemic and reperfused hearts and recovery of mechanical function of these hearts on reperfusion was studied. Isolated rat hearts were exposed to global ischemia for periods up to 60 min under various conditions of coronary flow, O2 supply, and fatty acid concentrations and were then reperfused for either 15 or 30 min under aerobic conditions both with and without fatty acids present. Tissue levels of ATP, creatine phosphate, long-chain acyl CoA, and long-chain acyl carnitine were determined at the end of the ischemic and reperfusion periods. In some experiments K+ arrest during ischemia was used to prevent adenine nucleotide depletion both in the absence and presence of high fatty acids. Although the ability of these hearts to recover their preischemic mechanical function varied from 8 to 90% and tissue levels of acyl CoA and acyl carnitine during ischemia varied from 3- to 10-fold depending on the condition, no correlation was found between the recovery of function during reperfusion and either the presence of fatty acid or high levels of tissue long-chain acyl CoA and carnitine esters during ischemia.