Enhanced ethanol production from sugarcane juice by galactose adaptation of a newly isolated thermotolerant strain of Pichia kudriavzevii
Enhanced ethanol production from sugarcane juice by galactose adaptation of a newly isolated thermotolerant strain of Pichia kudriavzevii
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DOI：
10.1016/j.biortech.2011.02.015
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发表时间：
2011-05-01
影响因子：
11.4
通讯作者：
Uppal, Satinder Kaur
中科院分区：
文献类型：
--
作者：
Dhaliwal, Sandeep Singh;Oberoi, Harinder Singh;Uppal, Satinder Kaur
经分子鉴定，从甘蔗汁中分离到的耐热酵母菌为东方巴氏毕赤酵母(Issatchenkia Orientalis)。适应于半乳糖培养的P.kudriavzevii细胞从甘蔗汁中产生的乙醇比不适应的细胞多30%左右。回收的电池可以连续使用四个周期，乙醇产量不会有明显下降。在实验室发酵罐中，以半乳糖适应的P.kudriavzevii细胞在40℃下发酵，由约14%(w/v)蔗糖、2%(w/v)葡萄糖和1%(w/v)果糖组成的甘蔗汁，酒精浓度和产率分别为71.9g L-1和4.0g L-1h(-1)。发酵过程中除乙醇外，还能产生3.30g L-1阿拉伯醇和4.19g L-1甘油，而不生成山梨醇和木糖醇。利用半乳糖适应的P.kudriavzevii细胞从甘蔗汁中生产乙醇具有扩大研究的潜力。(C)2011爱思唯尔有限公司。保留所有权利。
The thermotolerant yeast strain isolated from sugarcane juice through enrichment technique was identified as a strain of Pichia kudriavzevii (Issatchenkia orientalis) through molecular characterization. The P. kudriavzevii cells adapted to galactose medium produced about 30% more ethanol from sugarcane juice than the non-adapted cells. The recycled cells could be used for four successive cycles without a significant drop in ethanol production. Fermentation in a laboratory fermenter with galactose adapted P. kudriavzevii cells at 40 degrees C resulted in an ethanol concentration and productivity of 71.9 g L-1 and 4.0 g L-1 h(-1), respectively from sugarcane juice composed of about 14% (w/v) sucrose, 2% (w/v) glucose and 1% (w/v) fructose. In addition to ethanol, 3.30 g L-1 arabitol and 4.19 g L-1 glycerol were also produced, whereas sorbitol and xylitol were not formed during fermentation. Use of galactose adapted P. kudriavzevii cells for ethanol production from sugarcane juice holds potential for scale-up studies. (C) 2011 Elsevier Ltd. All rights reserved.