The effect of antecedent acute ethanol ingestion on the pancreas ultrastructure in taurocholate pancreatitis in rats
The effect of antecedent acute ethanol ingestion on the pancreas ultrastructure in taurocholate pancreatitis in rats
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DOI：
10.1006/exmp.1998.2226
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发表时间：
1998-10-01
影响因子：
3.6
通讯作者：
Jurkowska, G
中科院分区：
文献类型：
--
作者：
Andrzejewska, A;Dlugosz, JW;Jurkowska, G
在（1）牛磺胆酸盐诱导的急性胰腺炎后6、12和18 h，以及（2）胰腺炎前6 h急性胃内摄入40%乙醇（5 g/kg b.w.）后，检查大鼠胰腺超微结构。在相同的时间间隔测定胰腺特异性胰蛋白酶活性和血浆醇-淀粉酶。先前的急性乙醇摄入导致胰腺超微结构的改变明显加重。与未摄入乙醇的急性胰腺炎相比，摄入乙醇的急性胰腺炎导致酶原颗粒数量增加，RER通道分布更不规则，自噬体更丰富，线粒体变性更先进。在所有胰腺炎组中，胰蛋白酶活性在乙醇前均升高，但仅在18 h后才有统计学意义（P < 0.01）。胰腺炎诱导后12小时，这些形态学（而非生化）差异发生进展。急性胰腺炎18小时后，酶原颗粒的数量减少，在以前的酒精大鼠，但胰蛋白酶活性仍然是两倍高，在动物中没有给予乙醇。其他细胞损伤的迹象在酗酒大鼠中仍然更为突出。所获得的结果表明，即使是单一的急性酒精滥用急性胰腺炎之前，也会加重在这种疾病中观察到的形态学和生化病变，可能对预后产生负面影响。(C)北京：科学出版社.
The rat pancreas ultrastructure was examined 6, 12, and 18 h after (1) taurocholate-induced acute pancreatitis and after (2) pancreatitis preceded 6 h earlier by intragastric acute 40% ethanol ingestion (5 g/kg b.w.). Pancreatic specific trypsin activity and plasma ol-amylase were assayed at the same time intervals. The antecedent acute ethanol ingestion resulted in the evident aggravation of pancreas ultrastructural alterations. Acute pancreatitis preceded by ethanol resulted in the increase of zymogen granules number, RER channels were more irregularly distributed, autophagosomes were more abundant and degeneration of mitochondria was more advanced when compared to acute pancreatitis without ethanol ingestion. Tryptic activity increased to higher degree in all pancreatitis groups preceded by ethanol, but this difference was statistically significant (P < 0.01) only after 18 h. These morphological (but not biochemical) differences progressed 12 h after pancreatitis induction. After 18 h of acute pancreatitis the number of zymogen granules decreased in previously alcoholized rats, but tryptic activity remained twofold higher that in animals not given ethanol. Other signs of cellular impairment were still more prominent in alcoholized rats. The obtained results suggest that even single acute ethanol abuse prior to acute pancreatitis does aggravate the morphological and biochemical lesions observed in this disease with possible negative consequences for the prognosis. (C) 1998 Academic Press.