ON SIZING AND COUNTING OF MICROBUBBLES USING OPTICAL MICROSCOPY
ON SIZING AND COUNTING OF MICROBUBBLES USING OPTICAL MICROSCOPY
复制标题
DOI：
10.1016/j.ultrasmedbio.2010.09.004
复制
发表时间：
2010-12-01
影响因子：
2.9
通讯作者：
Eckersley, Robert J.
中科院分区：
文献类型：
--
作者：
Sennoga, Charles A.;Mahue, Veronique;Eckersley, Robert J.
使用光学显微镜（OM）评估了观察者内部和观察者之间（n = 3）微泡大小和计数的可变性。该系统使用标准化的单分散和多分散微球进行校准。校准结果显示，单分散微球的观察者内计数变化(C) = 13.0%，算术平均大小(MS) = 0.2%，观察者间C = 18.4%, MS = 0.6%。对于多分散微球，观察者内变化为：C = 6.9%, MS = 0.8%；观察者间变化为：C = 10.5%, MS = 0.3%。对于SonoVue (TM)，观察者内变异为：C = 23.3%, MS = 8.0%；观察者间变异为C = 6.8%, MS = 3.8%。结果表明，SonoVue （TM）的观察者内变化值较高是由于微泡随时间的自然衰减。本文提供了一个详细的协议，并概述了我们的方法中的潜在缺陷。这些结果与先前报道的结果基本一致，并与已知的尺寸分布相比较。（E-mail: c.sennoga@imperial.ac.uk） (C) 2010年世界超声医学与生物学联合会。
Intra- and interobserver (n = 3) variability of sizing and counting microbubbles using optical microscopy (OM) was assessed. The system was calibrated using standardised mono-disperse and poly-disperse microspheres. Results of the calibration show intraobserver variations of number count (C) = 13.0% and arithmetic mean size (MS) = 0.2%, and interobserver variations of C = 18.4% and MS = 0.6%, for the mono-disperse microspheres. For the poly-disperse microspheres, intraobserver variations were: C = 6.9% and MS = 0.8%, and interobserver: C = 10.5% and MS = 0.3%. For SonoVue (TM) the intraobserver variations were: C = 23.3% and MS = 8.0%, and interobserver C = 6.8% and MS = 3.8%. The results suggest that the higher values of the intraobserver variation for SonoVue (TM) arise from the natural decay of microbubbles over time. This article presents a detailed protocol and outlines potential pitfalls in our approach. These results are in general agreement with those previously reported and compare well with known size distributions. (E-mail: c.sennoga@imperial.ac.uk) (C) 2010 World Federation for Ultrasound in Medicine & Biology.