Enzyme immunoassay of blasticidin S with high sensitivity: a new and convenient method for preparation of immunogenic (hapten-protein) conjugates.
Enzyme immunoassay of blasticidin S with high sensitivity: a new and convenient method for preparation of immunogenic (hapten-protein) conjugates.
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高灵敏度杀稻瘟菌素 S 酶联免疫分析：一种制备免疫原性（半抗原-蛋白）缀合物的便捷新方法。
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
H. Munechika
中科院分区：
文献类型：
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作者：
T. Kitagawa;T. Kawasaki;H. Munechika
以N-(间马来酰亚胺)琥珀酰亚胺为交联剂，将水稻稻瘟病有效抗生素S与牛血清白蛋白偶联，制备了一种简便、新颖的偶联物，经硼氢化钠还原二硫键后与牛血清白蛋白偶联，制备了抗S抗体。BLS-MBS-BSA结合物每个BSA分子含有约16个BLS。利用另一种交联剂N-(马来酰亚胺丁氧基)琥珀酰亚胺，利用我们去年介绍的一种简便的标记方法，用β-D-半乳糖苷酶标记BLS。用标记的BLS和抗BLS抗血清建立了BLS的双抗体酶免疫测定法，每管BLS的最低检测浓度可达100pg。各种常用药物在该免疫检测中几乎没有反应性，表明所制备的抗BLS具有很高的特异性。该抗BLS抗体效价高，1000万倍稀释液可与酶标记的BLS结合。
An antibody against blasticidin S (BLS), an antibiotic effective for blast disease of rice, has been produced in rabbits immunized with a blasticidin S-protein conjugate prepared by a novel and convenient procedure devised to couple BLS to bovine serum albumin (BSA) after sodium borohydride reduction of its disulfide bonds, using N-(m-maleimidobenzoyloxy)succinimide (MBS) as a cross-linker. BLS-MBS-BSA conjugate contained about 16 BLS per BSA molecule. Enzyme labeling of BLS with beta-D-galactosidase was performed by utilizing another cross-linker, N-(gamma-maleimidobutyryloxy)succinimide by means of a convenient labeling method which we introduced last year. A double antibody enzyme immunoassay of BLS which could determine as little as 100 pg per tube of BLS was developed using labeled BLS and anti-BLS antiserum. Various commonly used drugs were found to have little reactivity in this immunoassay, indicating that the anti-BLS produced is highly specific. The titer of the anti-BLS was excellent and 10,000,000-fold diluted solution could bind with the enzyme labeled BLS.