Characterization of dentin matrix protein 1 gene in crocodilia
Characterization of dentin matrix protein 1 gene in crocodilia
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DOI：
10.1016/s0378-1119(99)00195-x
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发表时间：
1999-07-08
期刊：
影响因子：
3.5
通讯作者：
Klein, J
中科院分区：
文献类型：
--
作者：
Toyosawa, S;O'hUigin, C;Klein, J
以鸭嘴兽DMP 1基因为探针，从凯门鳄牙齿文库中筛选到几个含DMP 1基因的克隆。凯门鳄的DMP 1显示很少的氨基酸序列相似性，哺乳动物的DMP 1的大部分长度。然而，可以鉴定出一些高度保守的区域，它们对应于相应哺乳动物基因的缓慢进化部分。使用包含保守区域或基因的较长片段的探针的Southern印迹分析表明仅存在单个DMP 1基因座。在编码区，外显子-内含子边界和阅读框架是共同的凯门鳄和哺乳动物的基因，除了外显子1和5，这是更长的凯门鳄。哺乳动物和凯门鳄共享最后一个外显子的重复序列，由于高比例的Asp和Glu残基，因此具有高Ser含量和酸性。保守的哺乳动物细胞附着信号Arg-Gly-Asp在凯门鳄DMP 1中不存在。与釉原蛋白基因相反，DMP 1基因似乎在脊椎动物中迅速进化。(C)1999 Elsevier Science B. V.保留所有权利。
Several clones containing DMP1 cDNA were isolated from a caiman tooth library by screening with a platypus DMP1 probe. The caiman DMP1 shows little amino acid sequence similarity to mammalian DMP1s for much of its length. A few highly conserved regions can, however, be identified that correspond to the slowly evolving parts of the corresponding mammalian genes. Southern blot analysis using probes comprising either conserved regions or longer segments of the gene indicates that only a single DMP1 locus exists. In coding regions, exon-intron boundaries and reading frames are shared by caiman and mammalian genes with the exception of exons 1 and 5, which are longer in the caiman. The repetitive sequence of the last exon is shared by mammals and caiman as are the high Ser content and acidity due to a high proportion of Asp and Glu residues. The conserved mammalian cell-attachment signal Arg-Gly-Asp is absent in the caiman DMP1. In contrast to the amelogenin gene, the DMP1 gene appears to evolve rapidly in vertebrates. (C) 1999 Elsevier Science B.V. All rights reserved.