C-TAK1 interacts with microphthalmia-associated transcription factor, Mitf, but not the related family member Tfe3.
C-TAK1 interacts with microphthalmia-associated transcription factor, Mitf, but not the related family member Tfe3.
复制标题
C-TAK1 与小眼症相关转录因子 Mitf 相互作用，但不与相关家族成员 Tfe3 相互作用。
DOI：
10.1016/j.bbrc.2010.03.034
复制
发表时间：
2010
影响因子：
3.1
通讯作者：
Mansky,KimC
中科院分区：
文献类型：
--
作者：
Schwarz,Toni;Murphy,Sharlene;Sohn,Chee;Mansky,KimC
Microphthalmia-associated transcription factor, Mitf, has been shown to be necessary for regulating genes involved in osteoclast differentiation. Previously it was shown by others that Mitf translocates from the cytoplasm to the nucleus upon M-CSF/RANKL signaling in osteoclasts. Mitf’s movement is regulated by its interaction with 14-3-3 and the kinase C-TAK1. Here we demonstrate that the related family member, Tfe3, does not shuttle from the cytoplasm to the nucleus and does not interact with C-TAK1. We also demonstrate that overexpression of C-TAK1 inhibits the expression of Acp5 while a kinase dead C-TAK1 or a Mitf mutant that cannot interact with C-TAK1 increased expression of Acp5. Finally, we show that the catalytic subunit of protein phosphatase 2A is up-regulated in osteoclasts with M-CSF/RANKL signaling, indicating a possible mechanism for dephosphorylating Mitf on its 14-3-3 binding site and allowing Mitf to be translocated to the nucleus of osteoclasts.
