Expression of Distinct Self-Incompatibility Specificities in Arabidopsis thaliana
Expression of Distinct Self-Incompatibility Specificities in Arabidopsis thaliana
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DOI：
10.1534/genetics.109.102442
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发表时间：
2009-08-01
期刊：
影响因子：
3.3
通讯作者：
Nasrallah, June B.
中科院分区：
文献类型：
--
作者：
Boggs, Nathan A.;Dwyer, Kathleen G.;Nasrallah, June B.
物种内的平衡选择和物种间的快速基因进化的相互作用，可以混淆我们的能力，以确定种间和属间的等位基因对生殖的功能等效性。在十字花科植物中，被称为自交不亲和性（SI）的自交受精障碍中的交配特异性由两种高度多态性和共进化的蛋白质，S-位点受体激酶（SRK）及其S-位点富含半胱氨酸（SCR）配体之间的等位基因特异性相互作用控制。这些蛋白质在物种内和物种之间都是多样化的，使得通常难以单独从序列信息确定它们是否编码相同或不同的SI特异性。自花可育的拟南芥是通过自交不亲和性的丧失（通常在卷曲、连接处）和SRK或SCR的失活而从专性远交祖先进化而来的。然而，A.拟南芥在转化时表达自交不亲和性，所有SRK-SCR基因对都是从其自交不亲和近缘种A.抒情琴。在这里，我们表明，一些额外的和高度分歧SRK/SCR基因从A，天琴和另一种十字花科植物，大花荠，赋予自交不亲和A。thaliana中，或者作为从基因组文库分离的完整基因，或者在操作以产生嵌合融合体之后。我们描述了如何使用这种新开发的嵌合蛋白质策略，使我们能够测试SRK/SCR基因对从不同的类群的功能等效性，并测定内源性A。thaliana SRK和SCR序列。
The interplay of balancing selection within a species and rapid gene evolution between species can confound our ability to determine the functional equivalence of interspecific and intergeneric pairs of alleles underlying reproduction. In crucifer plants, matting specificity in the barrier to self-fertilization called self-incompatibility (SI) is controlled by allele-specific interactions between two highly polymorphic and co-evolving proteins, the S-locus receptor kinase (SRK) and its S-locus cysteine rich (SCR) ligand. These proteins have diversified both within and between species such that it is often difficult to determine from sequence information alone if they encode the same or different SI specificity. The self-fertile Arabidopsis thaliana was derived from an obligate outbreeding ancestor by loss of self-incompatibility, often in coil, junction with inactivation of SRK or SCR. Nevertheless, some accessions of A. thaliana call express self-incompatibility upon transformation with all SRK-SCR gene pair isolated front its self-incompatible close relative A. lyrata. Here we show that several additional and highly diverged SRK/SCR genes from A, lyrata and another crucifer plant, Capsella grandiflora, confer self-incompatibility in A. thaliana, either as intact genes isolated from genomic libraries or after manipulation to generate chimeric fusions. We describe how the use of this newly developed chimeric protein strategy has allowed us to test the functional equivalence of SRK/SCR gene pairs from different taxa and to assay the functionality of endogenous A. thaliana SRK and SCR sequences.