Nitric oxide modulates respiratory-related neural activity in the isolated brainstem of the bullfrog.
Nitric oxide modulates respiratory-related neural activity in the isolated brainstem of the bullfrog.
复制标题
一氧化氮调节牛蛙离体脑干中与呼吸相关的神经活动。
DOI：
10.1016/s0304-3940(98)00485-6
复制
发表时间：
1998
影响因子：
2.5
通讯作者：
Pacheco,JL
中科院分区：
文献类型：
--
作者：
Hedrick,MS;Morales,RD;Parker,JM;Pacheco,JL
以牛蛙(Rana Catesbeiana)脑干标本为材料，研究了一氧化氮(NO)对呼吸相关神经活动的影响。加入NO供体硝普钠(SNP)或NO合成的氨基酸前体L-精氨酸(L-Arg)可显著增加呼吸相关的猝发频率。非选择性一氧化氮合酶抑制剂7-硝基吲唑(7-ω-NI，7-NI)对一氧化氮合酶(NOS)的抑制作用可逆性地取消。这些结果表明，NO的产生可能通过神经元型一氧化氮合酶(NNOS)，为两栖类脑干的呼吸中枢模式生成器(CPG)提供了易化输入。内源性NO的产生可能是牛蛙中枢呼吸驱动到运动效应器的神经传递和/或神经调节所必需的细胞间或细胞内信使。
The effects of nitric oxide (NO) on respiratory-related neural activity were investigated using the isolated brainstem preparation from bullfrogs (Rana catesbeiana). Addition of the NO donor, sodium nitroprusside (SNP), or the amino acid precursor for NO synthesis, l-arginine (l-Arg), produced significant increases in respiratory-related burst frequency. Inhibition of nitric oxide synthase (NOS) with Nω-nitro-l-arginine (l-NA), a non-selective NOS inhibitor, 7-nitro indazole (7-NI), reversibly abolished burst activity. These results suggest that production of NO, probably via neuronal NOS (nNOS), provides a facilitatory input to the respiratory central pattern generator (CPG) in the amphibian brainstem. Endogenous production of NO may be a necessary inter- or intracellular messenger for neurotransmission and/or neuromodulation of central respiratory drive to motor effecters in the bullfrog.