Correlation among clinicopathological parameters of myocardial damage in rats treated with isoproterenol
Correlation among clinicopathological parameters of myocardial damage in rats treated with isoproterenol
复制标题
DOI：
10.1538/expanim.56.57
复制
发表时间：
2007-01-01
影响因子：
2.4
通讯作者：
Shirai, Norimitsu
中科院分区：
文献类型：
--
作者：
Kurata, Masaaki;Iidaka, Takeshi;Shirai, Norimitsu
研究了大鼠单次皮下注射0（生理盐水）、0.04、0.4和4 mg/kg异丙肾上腺素后心肌损伤的临床病理参数之间的相关性。给药后4 h测定总乳酸脱氢酶（LDH）、肌酸激酶（CK）及其同工酶（LDH-1、LDH-2和CK-MB）以及肌钙蛋白I和托宁蛋白T。使用Bayer Centaur和DPC Immulyze通过化学发光法以及ELISA测定肌钙蛋白I。使用Trop T Sensitive半定量测定肌钙蛋白T。LDH同工酶与肌钙蛋白I（Centaur）和肌钙蛋白T之间存在高度相关性。本研究结果为解释毒性研究中不同方法测定的心肌损伤不同参数的变化提供了基线。
The correlation among clinicopathological parameters of myocardial damage was investigated in rats administered a single subcutaneous dose of isoproterenol at 0 (saline), 0.04, 0.4 and 4 mg/kg. Total lactate dehydrogenase (LDH), creatine kinase (CK), and their isoenzymes (LDH-1, LDH-2 and CK-MB), as well as troponin I and tropinin T, were measured 4 h after the administration of the drug. Troponin I was determined by a chemiluminescence method using Bayer Centaur and DPC Immulyze, as well as by ELISA. Troponin T was assayed semi-quantitatively using Trop T Sensitive. A high correlation was found among LDH isoenzymes, troponin I (Centaur) and troponin T. The present result provides a baseline for interpreting changes in the different parameters of myocardial damage assayed by different methods in toxicity studies.