Expression of TGF-beta s and TGF-beta type II receptor mRNAs in mouse folliculogenesis: stored maternal TGF-beta 2 message in oocytes.
Expression of TGF-beta s and TGF-beta type II receptor mRNAs in mouse folliculogenesis: stored maternal TGF-beta 2 message in oocytes.
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小鼠卵泡发生中 TGF-β 和 TGF-β II 型受体 mRNA 的表达：卵母细胞中储存的母体 TGF-β 2 信息。
DOI：
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发表时间：
1994
影响因子：
3.1
通讯作者：
G. Bilbe
中科院分区：
文献类型：
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作者：
P. Schmid;D. Cox;H. van der Putten;G. Mcmaster;G. Bilbe
本原位杂交研究描述了TGF-β 1，TGF-β 2，TGF-β 3和TGF-β II型受体mRNA在卵泡发育过程中的表达，以及TGF-β 2转录物在早期植入前胚胎中的时间模式和丰度。TGF-β 1杂交信号在窦后卵泡和黄体的外颗粒细胞层中最突出，而TGF-β II型受体mRNA的强表达仅限于卵泡膜细胞。在窦前和窦后卵泡的所有主要细胞类型中观察到弱TGF-β 3 mRNA表达。最值得注意的是，在发育中的卵母细胞中检测到的非常强的TGF-β 2杂交信号在受精后迅速下降。虽然在两细胞胚胎中仍然丰富，但在四细胞和八细胞胚胎中几乎检测不到TGF-β 2杂交信号。这些发现，以及TGF-β 2 mRNA 3'非翻译区中存在的特定序列基序，表明TGF-β 2转录本是作为母体信息储存的。
The present in situ hybridisation study describes expression of TGF-beta 1, TGF-beta 2, TGF-beta 3, and TGF-beta type II receptor mRNA during follicular development, and the temporal pattern and abundance of TGF-beta 2 transcripts in early pre-implantation embryos. TGF-beta 1 hybridisation signals were most prominent in the outer granulosa cell layers of the post-antral follicles and in the corpus luteum, whereas strong expression of TGF-beta type II receptor mRNA was confined to thecal cells. Weak TGF-beta 3 mRNA expression was observed in all major cell types of pre- and post-antral follicles. Most notably, the very strong TGF-beta 2 hybridisation signals which were detected in developing oocytes declined rapidly following fertilization. Although still abundant in two-cell embryos, TGF-beta 2 hybridisation signals were barely detectable in four- and eight-cell embryos. These findings, and the presence of specific sequence motifs in the 3' untranslated region of TGF-beta 2 mRNAs, suggest that TGF-beta 2 transcripts are stored as maternal messages.