Bilateral renal cryodenervation decreases arterial pressure and improves insulin sensitivity in fructose-fed Sprague-Dawley rats.
Bilateral renal cryodenervation decreases arterial pressure and improves insulin sensitivity in fructose-fed Sprague-Dawley rats.
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双侧肾冷冻去神经术可降低果糖喂养的 Sprague-Dawley 大鼠的动脉压并提高胰岛素敏感性。
DOI：
10.1152/ajpregu.00020.2018
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Rossi,NoreenF
中科院分区：
文献类型：
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作者：
Soncrant,Tyler;Komnenov,Dragana;Beierwaltes,WilliamH;Chen,Haiping;Wu,Min;Rossi,NoreenF
现代饮食中普遍食用高果糖食物。研究表明，适度高果糖摄入与高盐饮食相结合一周会导致血压升高，但无法抑制血浆肾素活性（PRA）。我们检验了高血压和高 PRA 是肾交感神经活动 (RSNA) 升高的结果这一假设。在方案 1 中，我们通过遥测法对清醒的 Sprague-Dawley 大鼠进行 RSNA 评估，这些大鼠在 0.4% NaCl 饮食 (NS) 的饮用水中添加 20% 果糖或 20% 葡萄糖，持续 1 周，然后过渡到 4% NaCl 饮食 (HS)。再过一周后，果糖喂养的 HS 大鼠的平均动脉压 (MAP) 和 RSNA 显着增加，而葡萄糖喂养的 HS 大鼠则没有显着增加。在方案 2 中，以 NS 或 HS 饮食喂养果糖 (Fruc) 或葡萄糖 (Glu) 的大鼠 3 周，进行假去神经术 (shamDNX) 或使用冷冻消融 (cryoDNX) 进行双侧肾脏去神经术。与 Glu-NS、Glu-HS 或 Fruc-NS 大鼠相比，Fruc-HS 大鼠的 MAP 较高，而冷冻 DNX 后则降低（P < 0.01）。 Fruc-HS shamDNX 大鼠中 MAP 没有变化。 CryoDNX 中肾脏去甲肾上腺素含量降低了 85%（与 shamDNX 相比，P< 0.01）。在 Fruc-NS 和 Fruc-HS 大鼠中，cryoDNX 后 PRA 显着下降。四组之间的非空腹血糖水平相似。 Fruc-HS CryoDNX 大鼠的葡萄糖与胰岛素比率显着增加，这与更高的胰岛素敏感性一致。总而言之，这些研究表明，在喂食适度高果糖饮食短短 3 周的大鼠中，肾交感神经兴奋至少部分地导致了盐依赖性 MAP 增加、PRA 增加和胰岛素敏感性降低。
Consumption of food high in fructose is prevalent in modern diets. One week of moderately high fructose intake combined with high salt diet has been shown to increase blood pressure and failed to suppress plasma renin activity (PRA). We tested the hypothesis that the hypertension and high PRA are consequences of elevated renal sympathetic nerve activity (RSNA). Inprotocol 1, we assessed RSNA by telemetry in conscious Sprague-Dawley rats given 20% fructose or 20% glucose in drinking water on a 0.4% NaCl diet (NS) for 1 wk and then transitioned to a 4% NaCl diet (HS). After an additional week, mean arterial pressure (MAP) and RSNA increased significantly in fructose-fed but not glucose-fed HS rats. Inprotocol 2, fructose (Fruc)- or glucose (Glu)-fed rats on NS or HS diet for 3 wk underwent sham denervation (shamDNX) or bilateral renal denervation using cryoablation (cryoDNX). MAP was higher in Fruc-HS rats compared with Glu-NS, Glu-HS, or Fruc-NS rats and decreased after cryoDNX (P< 0.01). MAP did not change in Fruc-HS shamDNX rats. Renal norepinephrine content decreased by 85% in cryoDNX (P< 0.01 vs. shamDNX). PRA significantly decreased after cryoDNX in both Fruc-NS and Fruc-HS rats. Nonfasting blood glucose levels were similar among the four groups. Glucose-to-insulin ratio significantly increased in Fruc-HS cryoDNX rats, consistent with greater insulin sensitivity. Taken together, these studies show that renal sympathoexcitation is, at least in part, responsible for salt-dependent increases in MAP, increased PRA, and decreased insulin sensitivity in rats fed a moderately high fructose diet for as little as 3 wk.