Genome Analysis of a Limnobacter sp. Identified in an Anaerobic Methane-Consuming Cell Consortium
Genome Analysis of a Limnobacter sp. Identified in an Anaerobic Methane-Consuming Cell Consortium
复制标题
沼杆菌属的基因组分析。
DOI：
10.3389/fmars.2016.00257
复制
发表时间：
2016-01-01
影响因子：
3.7
通讯作者：
Wang, Fengping
中科院分区：
文献类型：
--
作者：
Chen, Ying;Feng, Xiaoyuan;Wang, Fengping
Limnobacter属的物种广泛存在于各种环境中，但对其代谢潜力和环境适应机制的了解有限。在本研究中，从富集的厌氧甲烷氧化（AOM）微生物群落中捕获了含有Limnobacter和厌氧甲烷营养古细菌（ANME）的细胞聚集体。获得并分析了Limnobacter的基因组库，首次从AOM环境中了解Limnobacter的代谢。该Limnobacter被发现含有参与Embden-Meyerhof途径、柠檬酸循环、香茅醛降解和各种有机物质转运的基因，表明可能存在异养生活方式。在Limnobacter中发现了一些参与硫氧化、氧化磷酸化和乙醇发酵的基因，这些基因同时服务于有氧和厌氧目的。本研究表明，在AOM环境中，Limnobacter菌株可能以AOM活性产生的有机物为食，随后可能通过硫氧化提供硫酸盐为AOM群落做出贡献。
Species of Limnobacter genus are widespread in a variety of environments, yet knowledges upon their metabolic potentials and mechanisms of environmental adaptation are limited. In this study, a cell aggregate containing Limnobacter and anaerobic methanotrophic archaea (ANME) was captured from an enriched anaerobic methane oxidizing (AOM) microbial community. A genomic bin of Limnobacter was obtained and analyzed, which provides the first metabolic insights into Limnobacter from an AOM environment. This Limnobacter was found to contain genes involved in the Embden-Meyerhof pathway, the citrate cycle, citronellol degradation, and transporters of various organic substances, indicating a potentially heterotrophic lifestyle. A number of genes involved in sulfur oxidization, oxidative phosphorylation and ethanol fermentation that serve both aerobic and anaerobic purposes have been found in Limnobacter. This work suggests that in the AOM environment, Limnobacter strains may live on the organic substances produced through AOM activity and subsequently may contribute to the AOM community by providing sulfate from sulfur oxidation.