Secretion of cytokines and chemokines by polarized human epithelial cells from the female reproductive tract
Secretion of cytokines and chemokines by polarized human epithelial cells from the female reproductive tract
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DOI：
10.1093/humrep/deh806
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发表时间：
2005-06-01
影响因子：
6.1
通讯作者：
Wira, CR
中科院分区：
文献类型：
--
作者：
Fahey, JV;Schaefer, TM;Wira, CR
背景技术背景：吸引免疫细胞的促炎趋化因子和激活免疫细胞的细胞因子有助于女性生殖道的正常生理稳态，并且需要有效地处理潜在的病原微生物。粘液上皮细胞能够产生与先天性和适应性免疫系统的细胞通信的这些因子。方法：从输卵管，子宫内膜和子宫颈内膜上皮细胞分离和生长的高跨上皮阻力的细胞插入从七个病人子宫切除术。白细胞介素（IL）-8，IL-6，粒细胞集落刺激因子（G-CSF），单核细胞趋化蛋白-1（MCP-1），粒细胞-巨噬细胞集落刺激因子（GM-CSF），肿瘤坏死因子-α（TNF-α）和巨噬细胞炎性肽-1 β通过Luminex微珠分析或酶联免疫吸附试验（ELISA）评估MIP-1 β在来自顶侧和基底侧隔室的上皮细胞条件培养基中。研究结果：除MCP-1外，由极化上皮细胞组成性产生的7种趋化因子/细胞因子优先分泌于顶端。在所有病例中发现浓度模式，IL-8和IL-6产生量最大。结论：IL-8、IL-6、G-CSF和MCP-1的浓度与感染患者生殖道液中发现的水平相似。大量生物活性趋化因子和细胞因子的组成性分泌和区室化为上皮细胞在女性生殖道中作为先天免疫保护的守门人的作用提供了额外的证据。
BACKGROUND: Pro-inflammatory chemokines that attract and cytokines that activate immune cells contribute to normal physiological homeostasis in the female reproductive tract, and are needed to deal effectively with potential pathogenic microbes. Mucosal epithelial cells are capable of producing these factors that communicate with cells of the innate and adaptive immune systems. METHODS: Epithelial cells from Fallopian tube, endometrium and endocervix were isolated and grown to high transepithelial resistance in cell inserts from seven patients who had hysterectomies. Interleukin (IL)-8, IL-6, granulocyte colony-stimulating factor (G-CSF), monocyte chemoattractant protein-1 (MCP-1), granulocyte-macrophage colony-stimulating factor (GM-CSF), tumour necrosis factor-alpha (TNF-alpha) and macrophage inflammatory peptide-1 beta (MIP-1 beta) were assessed by Luminex bead analysis or enzyme-linked immunosorbent assay (ELISA) in epithelial cell conditioned media from the apical and basolateral compartments. RESULTS: With the exception of MCP-1, the seven chemokines/cytokines constitutively produced by the polarized epithelial cells were preferentially secreted apically. A concentration pattern was found in all cases, with IL-8 and IL-6 produced in the greatest quantity. CONCLUSIONS: The concentrations of IL-8, IL-6, G-CSF and MCP-1 are similar to the levels found in reproductive tract fluids of patients with infection. The constitutive secretion and compartmentalization of large quantities of bioactive chemokines and cytokines provide additional evidence for the role of epithelial cells as gatekeepers of innate immune protection in the female reproductive tract.