Germ-line chimaeras can produce both strains of fowl with high efficiency after partial sterilization.
Germ-line chimaeras can produce both strains of fowl with high efficiency after partial sterilization.
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种系嵌合体在部分绝育后可以高效地产生这两种家禽品系。
DOI：
10.1530/jrf.0.0980637
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
K. Simkiss
中科院分区：
文献类型：
--
作者：
L. Vick;G. Luke;K. Simkiss
已知药物布舒坦对迁徙的原始生殖细胞(PGCs)具有细胞毒性。将40微升芝麻油中的0、25、50和250微克的丁硫丹注入孵化0、24、48和72h的白来航蛋的卵黄中，观察胚胎的存活率，结果表明，注入时年龄越大的胚胎存活率越高。增加丁硫丹的剂量会降低小鼠的存活率。随着丁硫丹剂量的增加，胚胎出现异常的比例也随之增加。对16天胚胎性腺组织切片中生殖细胞的数量进行了估计，50微克和250微克白头翁处理组的生殖细胞数量显着低于对照组。在24-30小时内注射50微克的丁硫丹，在50-55小时内向胚胎血管内注射含有罗德岛红胚性生殖细胞的生发新月细胞悬液。从这些实验中孵化出来的20只幼崽被培养到性成熟。所有这些鸟都是可繁殖的，一半的繁殖群体从转移的生殖细胞中产生后代的比率约占总数的35%。这项技术将提高生产转基因配子的效率。
The drug busulphan is known to be cytotoxic to migrating primordial germ cells (PGCs). A technique is described in which doses of 0, 25, 50 and 250 micrograms busulphan in 40 microliters sesame oil were injected into the yolk of White Leghorn eggs incubated for 0, 24, 48 and 72 h. The percentage survival values of these embryos showed that the older the embryo at the time of injection, the greater the survival. Increasing the dose of busulphan decreased the survival. The percentage of embryos showing abnormalities increased with higher doses of busulphan. The number of germ cells in histological sections from gonads of 16-day embryos was estimated and in embryos treated with 50 micrograms and 250 micrograms busulphan the number of germ cells was significantly less than in the controls. Eggs were injected with 50 micrograms busulphan at 24-30 h, and at 50-55 h the embryos received an intravascular injection of a germinal crescent cell suspension containing PGCs from Rhode Island Red embryos. Twenty hatchlings from these experiments were raised to sexual maturity. All these birds were fertile and half of the breeding groups producing offspring from the transferred germ cells at a rate of about 35% of the total. The technique would improve the efficiency of producing transgenic gametes.