Pseudomonas stutzeri ferredoxin: close similarity to Azotobacter vinelandii and Pseudomonas ovalis ferredoxins.

Pseudomonas stutzeri ferredoxin: close similarity to Azotobacter vinelandii and Pseudomonas ovalis ferredoxins.
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施氏假单胞菌铁氧还蛋白:与维氏固氮菌和卵形假单胞菌铁氧还蛋白非常相似。

DOI:
10.1093/oxfordjournals.jbchem.a122450
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发表时间:
1988
影响因子:
2.7
通讯作者:
H. Matsubara
H. Matsubara
中科院分区:
生物学4区
文献类型:
--
作者:
K. Saeki;S. Wakabayashi;W. Zumft;H. Matsubara

文献摘要

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通过蛋白酶消化、Edman降解和羧肽酶消化,确定了菌株ZoBell铁氧化还蛋白的完整初级结构为:TFVVTDNCIKCKYTDCVEVCPVDCFYEGPNFLVIH pdecidcalcepecpaqaifsedevpedqqefieldlaevwpnite kkdaladaeewdgkkdklqyler。计算出的分子量为12,110,不包括铁原子和硫原子。氨基酸序列与黄氏固氮菌和卵状假单胞菌高度同源。与其他两个一样,它显示在第二和第三个Cys之间有一个tyrr - thr插入,在第24位有额外的Cys,与梭状芽孢杆菌型铁氧毒素相比,它有一个延长的c端序列。
The complete primary structure of Pseudomonas stutzeri strain ZoBell ferredoxin was determined by a combination of protease digestion, Edman degradation, and carboxypeptidase digestion and was: TFVVTDNCIKCKYTDCVEVCPVDCFYEGPNFLVIH PDECIDCALCEPECPAQAIFSEDEVPEDQQEFIELNADLAEVWPNITE KKDALADAEEWDGVKDKLQYLER. The calculated molecular weight was 12,110 excluding iron and sulfur atoms. The amino acid sequence was highly homologous to those of Azotobacter vinelandii and Pseudomonas ovalis ferredoxins. It showed, like the other two, a Tyr-Thr insertion between the second and third Cys, and extra Cys at position 24 and, compared to Clostridium- and Bacillus-type ferredoxins, an extended C-terminal sequence.