The human placental growth hormone variant is mitogenic for rat lymphoma Nb2 cells.
The human placental growth hormone variant is mitogenic for rat lymphoma Nb2 cells.
复制标题
人胎盘生长激素变体对大鼠淋巴瘤 Nb2 细胞具有促有丝分裂作用。
作者：
B. Nickel;Elissavet Kardamij;Peter A. Cattin
虽然有证据表明人胎盘生长激素变异体(hGH-V)具有促进生长的功能，但这种激素的催乳活性尚未被证明。稳定表达hGH-V基因的大鼠垂体前叶肿瘤细胞(GC[hGH-V]细胞)能合成和分泌hGH-V。该激素在核苷酸(大于90%)和氨基酸(大于80%)水平上与垂体型生长激素(hGH-N)和绒毛膜生长激素(HCS)有相当大的结构相似性。正如预期的那样，hGH-N和HCS抗体都通过免疫印迹检测hGH-V。然而，hGH-V，而不是hGH-N或HCS，与人或大鼠垂体催乳素(PRL)抗体发生交叉反应。这些数据表明，hGH-V和垂体PRL所共有的结构特征在hGH-N和HCS中不存在。氨基酸序列的比较表明，hGH-V和hPRL有两个共同的表位，hGH-N和HCS在结构上存在差异。在大鼠淋巴瘤NB2细胞生物实验中评估了hGH-V可能的产乳活性。来自GC[hGH-V]细胞的条件培养液允许乳素依赖性NB2淋巴瘤细胞在培养中生长。这种活性可被抗大鼠PRL的抗体阻断，但不能被hPRL或hGH-N阻断。HGH-V与hPRL、HCS、hGH-N等14种产乳激素的氨基酸序列比较发现，hGH-V有6个保守氨基酸。这些数据表明，在体内分泌的hGH-V蛋白具有产乳和促进生长的功能。
Although there is evidence that human (h) placental GH variant (hGH-V) possesses a growth-promoting function, lactogenic activity by the hormone has not been demonstrated. Rat anterior pituitary tumor (GC) cells stably transfected with the hGH-V gene (GC [hGH-V] cells) synthesize and secrete hGH-V. This hormone shares considerable structural similarity with pituitary growth hormone (hGH-N) and chorionic somatomammotropin (hCS) at the nucleotide (greater than 90%) and amino acid (greater than 80%) levels. As expected, both hGH-N and hCS antibodies detect hGH-V by immunoblotting. However, hGH-V, but not hGH-N or hCS, cross-reacts with human or rat pituitary prolactin (PRL) antibodies. These data indicate that structural features shared by hGH-V and pituitary PRL are not present in hGH-N or hCS. Comparison of amino acid sequences implicates two regions that may account for a common epitope between hGH-V and hPRL, and structural difference from hGH-N and hCS. The possible lactogenic activity by hGH-V was assessed in a rat lymphoma Nb2 cell bioassay. Conditioned medium from GC[hGH-V] cells permitted growth of lactogen-dependent Nb2 lymphoma cells in culture. This activity was blocked by antibodies raised to rat PRL but not hPRL or hGH-N. Comparison of the hGH-V amino acid sequence with those from 14 other lactogenic hormones, including hPRL, hCS and hGH-N, reveals 6 conserved amino acids. These data indicate a lactogenic as well as growth-promoting function for the secreted hGH-V protein in vivo.
当转染到大鼠垂体肿瘤细胞中时，人生长激素基因受到三碘甲状腺原氨酸的负调节。
DOI：
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发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Cattini,PA;Anderson,TR;Baxter,JD;Mellon,P;Eberhardt,NL
通讯作者：
Eberhardt,NL
人类胎盘中两种不同种类的人类生长激素变异体 mRNA 预测了新型生长激素蛋白的表达。
DOI：
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发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Cooke,NE;Ray,J;Emery,JG;Liebhaber,SA
通讯作者：
Liebhaber,SA