Molecular analysis of duplicated esterase genes in Drosophila melanogaster.
Molecular analysis of duplicated esterase genes in Drosophila melanogaster.
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果蝇中重复酯酶基因的分子分析。
DOI：
10.1093/oxfordjournals.molbev.a040582
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发表时间：
1990
影响因子：
10.7
通讯作者：
Richmond,RC
中科院分区：
文献类型：
--
作者：
Collet,C;Nielsen,KM;Russell,RJ;Karl,M;Oakeshott,JG;Richmond,RC
从果蝇DNA文库中分离出含有酯酶6（Est-6）cDNA克隆同源序列的基因组克隆。基因组和cDNA序列的比较显示，Est-6基因包括两个外显子，一个为1，387 bp，一个为248 bp，由一个51 bp的短内含子分隔。进一步的测序显示存在Est-6基因的串联重复（表示为Est-P），其也具有1，387 bp的外显子和248 bp的外显子，由56 bp的短内含子分开。这两个基因在DNA和蛋白质水平上的相似性分别为64%和60%。基因的编码区相隔197个碱基，并且推定的Est-P的5'调控序列至少与Est-6的3'非编码区重叠。转录同源EST-P中检测到晚期幼虫和成人的每一个性别，而EST-6转录本存在于所有的生命阶段，但主要是在成年男性。这表明两个基因的产物具有不同的生理功能。Southern和北方印迹杂交分析的20 kb区域周围的Est-6/Est-P重复未能检测到任何其他重复的酯酶基因，虽然这一地区是积极转录。
Genomic clones containing sequences homologous to an esterase 6 (Est-6) cDNA clone were isolated from a library of Drosophila melanogaster DNA. Comparison of the genomic and cDNA sequences revealed that the Est-6 gene comprises two exons, one of 1,387 bp and one of 248 bp, separated by a short intron of 51 bp. Further sequencing revealed the presence of a tandem duplication of the Est-6 gene (denoted Est-P) which also has an exon of 1,387 bp and an exon of 248 bp, separated by a short intron of 56 bp. The two genes show similarities of 64% and 60% at the DNA and protein levels, respectively. The coding regions of the genes are 197 bases apart, and presumptive 5' regulatory sequences of Est-P overlap at least the 3' noncoding region of Est-6. Transcripts homologous to Est-P were detected in late larvae and adults of each sex, whereas Est-6 transcripts are present in all life stages but are predominant in adult males. This suggests different physiological functions for the products of the two genes. Southern and Northern blot hybridization analyses of the 20-kb region surrounding the Est-6/Est-P duplication failed to detect any other duplicated esterase genes, although this region is actively transcribed.