Three-dimensional CaP/gelatin lattice scaffolds with integrated osteoinductive surface topographies for bone tissue engineering
Three-dimensional CaP/gelatin lattice scaffolds with integrated osteoinductive surface topographies for bone tissue engineering
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DOI：
10.1088/1758-5090/7/1/015005
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发表时间：
2015-03-01
期刊：
影响因子：
9
通讯作者：
Su, Bo
中科院分区：
文献类型：
--
作者：
Nadeem, Danish;Smith, Carol-Anne;Su, Bo
已知表面形貌影响干细胞，并且已被广泛用作物理刺激以调节细胞行为，包括2D表面上的粘附、增殖和分化。将明确定义的表面形貌整合到用于组织工程的三维（3D）支架中将有助于指导细胞命运用于预期应用。技术挑战仍然是如何从一系列可生物降解和生物相容性材料制造具有受控表面形貌的3D支架。在本文中，一种新的制造工艺，使用计算机数控加工和层压，使三维磷酸钙/明胶复合材料支架与集成的表面微图案，通过压花机加工前引入。几何分析表明，这种方法是通用的，可以用来制作各种各样的网格与孔隙率，满足骨组织工程的基本要求。在体外和体内的研究表明，微图案化的复合材料支架的表面包括40 μ m的凹坑和50 μ m的凹槽是最佳的改善成骨。结果表明，一种新的制造工艺的潜力，用于生产细胞指导支架与设计的表面形貌，以诱导特定的组织再生。
Surface topography is known to influence stem cells and has been widely used as physical stimuli to modulate cellular behaviour including adhesion, proliferation and differentiation on 2D surfaces. Integration of well-defined surface topography into three-dimensional (3D) scaffolds for tissue engineering would be useful to direct the cell fate for intended applications. Technical challenges are remaining as how to fabricate such 3D scaffolds with controlled surface topography from a range of biodegradable and biocompatible materials. In this paper, a novel fabrication process using computer numerically controlled machining and lamination is reported to make 3D calcium phosphate/gelatin composite scaffolds with integrated surface micropatterns that are introduced by embossing prior to machining. Geometric analysis shows that this method is versatile and can be used to make a wide range of lattices with porosities that meet the basic requirements for bone tissue engineering. Both in vitro and in vivo studies show that micropatterned composite scaffolds with surfaces comprising 40 mu m pits and 50 mu m grooves were optimal for improved osteogenesis. The results have demonstrated the potential of a novel fabrication process for producing cell-instructive scaffolds with designed surface topographies to induce specific tissue regeneration.