Abnormal immunoreactivity of the E-cadherin-catenin complex in gastric carcinoma: Relationship with patient survival
Abnormal immunoreactivity of the E-cadherin-catenin complex in gastric carcinoma: Relationship with patient survival
复制标题
DOI：
10.1016/s0016-5085(97)70218-x
复制
发表时间：
1997-01-01
期刊：
影响因子：
29.4
通讯作者：
Farthing, MJG
中科院分区：
文献类型：
--
作者：
Jawhari, A;Jordan, S;Farthing, MJG
背景和目标：E-cadherin-catenin复合物在维持正常组织结构中起关键作用，其任何组分的突变被认为导致细胞-细胞粘附的丧失并有助于肿瘤形成。本研究的目的是检测E-cadherin和α-、γ-和γ-catenin在胃癌和异型增生中的表达，并确定与肿瘤特征和生存的任何关系。使用89例胃癌、淋巴结转移和24例异型增生患者和10例健康对照的胃活检标本进行E-钙粘蛋白和α-、β-和γ-连环蛋白的免疫过氧化物酶染色。结果：在对照活检标本中观察到复合物的所有组分的膜染色。高达57%的胃异型增生和90%的肿瘤中钙粘蛋白-连环蛋白复合物的一种或多种成分染色异常，弥漫性肿瘤中E-钙粘蛋白和γ-连环蛋白染色异常的发生率高于肠肿瘤（分别为P < 0.0005和< 0.05）。未发现与肿瘤分级或分期相关，在肠和弥漫性肿瘤中，β-连环蛋白的膜表达具有生存优势，与肿瘤类型、分级或分期无关（P < 0.005），结论：E-cadherin-catenin复合体的异常表达常发生在胃癌中，与生存率低的密切相关性表明异常的β-连环蛋白可能是一个有用的预后标志物。
Background & Aims: The E-cadherin-catenin complex plays a critical role in the maintenance of normal tissue architecture, Mutation of any of its components is believed to result in loss of cell-cell adhesion and contribute to neoplasia, The aim of this study was to examine the expression of E-cadherin and alpha-, gamma-, and gamma-catenin ire gastric carcinoma and dysplasia and determine any relationship with tumor characteristics and survival, Methods: Immunoperoxidase staining of E-cadherin and alpha-, beta-, and gamma-catenin was performed using 89 gastric carcinomas, lymph node metastases, and gastric biopsy specimens from 24 patients with dysplasia and 10 healthy controls, Results: Membranous staining was observed in control biopsy specimens for all components of the complex. Up to 57% of gastric dysplasia and 90% of tumors stained abnormally for one or more components of the cadherin-catenin complex, Abnormal E-cadherin and gamma-catenin staining occurred more frequently in diffuse than intestinal tumors (P < 0.0005 and < 0.05, respectively). No association with tumor grade or stage was found, A survival advantage was noted in intestinal and diffuse tumors retaining membranous expression of beta-catenin, independent of tumor type, grade, or stage (P < 0.005), Conclusions: Abnormal expression of the E-cadherin-catenin complex occurs frequently in gastric carcinoma, The close correlation with poor survival suggests that abnormal beta-catenin may be a useful prognostic marker.