Compared Analysis on the Sequences of Mitochondrial CR Gene and COI Gene Fragments of Nine Wild Stocks of Portunus trituberculatus from the Marginal Seas of China
Compared Analysis on the Sequences of Mitochondrial CR Gene and COI Gene Fragments of Nine Wild Stocks of Portunus trituberculatus from the Marginal Seas of China
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发表时间：
2008
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通讯作者：
F. Bing
中科院分区：
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作者：
F. Bing
对中国野生三疣梭子蟹9个群体的线粒体CR基因和COⅠ基因片段进行了扩增和测序，获得了CR基因和COⅠ基因长分别为530bp和584bp的部分基因片段。CR基因和COⅠ基因的A+T平均含量分别为73.2%和62.2%。此外，从83份样品的91个变异点中鉴定出66种单倍型，从96份样品中鉴定出33种单倍型，共42个变异点。遗传距离和聚类分析表明，黄海、东海、中国、渤海、渤海、黄海、中国、渤海等6个种群的遗传距离最近而南中国海三个群体间的遗传距离最近，任意两个群体间的固定指数(Fst)在-0.074 54~0.270 87之间，有的达到显著或极显著水平，说明中国不同野生群体间存在一定的遗传分化。
Mitochondrial CR gene and COⅠ gene fragments were amplified and sequenced from 9 wild stocks of Portunus trituberculatus in China.Respectively,530 bp and 584 bp long partial gene fragments of the CR gene and the COⅠ gene were obtained.A+T mean contents of the CR sequences and the COⅠ sequences were respectively 73.2% and 62.2%.Moreover,66 haplotypes were identified from 83 samples with 91 variation sites of CR gene and 33 haplotypes were identified from 96 samples with 42 variation sites of COⅠ gene.The genetic distance and cluster analysis showed that genetic distances were nearest among six stocks of Yellow Sea,East China Sea,Bohai Sea,while the genetic distances were nearest among three stocks of South China Sea.The fixation indices (F st) were from -0.074 54 to 0.270 87 between any two stocks and some of them got to significant and great significant level.So these indicated that there were some genetic differentiations in different wild stocks of P.trituberculatus in China.