Expression of c-Met correlates with grade of malignancy in human astrocytic tumours: an immunohistochemical study
Expression of c-Met correlates with grade of malignancy in human astrocytic tumours: an immunohistochemical study
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DOI：
10.1046/j.1365-2559.1997.3010889.x
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发表时间：
1997-11-01
期刊：
影响因子：
6.4
通讯作者：
Koono, M
中科院分区：
文献类型：
--
作者：
Nabeshima, K;Shimao, Y;Koono, M
目的：最近的研究表明肝细胞生长因子/分散因子（HGF/SF）参与胶质瘤细胞侵袭和肿瘤进展，我们研究了星形胶质细胞肿瘤中表达c-Met蛋白（HGF/SF的细胞表面受体）的肿瘤细胞的分布和比例，还鉴定了肿瘤组织中表达c-Met的细胞类型。 43 例星形细胞瘤，包括 14 例低级别星形细胞瘤 (A)、13 例间变性星形细胞瘤 (AA) 和 16 例多形性胶质母细胞瘤 (GBM)。 c-Met 反应性主要在肿瘤细胞的细胞质中得到证实，奇异的大肿瘤细胞倾向于强烈染色。 (A) 中 21.4% 的 c-Met 表达水平较高（大于或等于 2+，超过 25% 细胞呈阳性），而 (AA) 中为 53.8%，(GBM) 中为 87.5%（P < 0.001），表明 c-Met 蛋白染色与高级星形细胞肿瘤之间存在密切关系。此外，c-Met 免疫反应性也显示在肿瘤微观中微血管系统。反应性星形胶质细胞和皮质中被神经胶质瘤细胞浸润的神经元。在 85.7% 含有浸润皮质的病例中，神经元呈阳性，而非肿瘤区域无神经元 (P < 0.002)。 结论：该证据表明大脑中 c-Met 的表达可能与星形细胞瘤进展和反应过程有关。对表达 c-Met 的细胞类型进行免疫组织化学测定有助于了解 c-Met 在肿瘤组织中的可能作用。
Aims: Recent studies suggest the involvement of hepatocyte growth factor/scatter factor (HGF/SF) in glioma cell invasion and tumour progression, We investigated the distribution and rate of tumour cells that express c-Met protein, which is the cell-surface receptor for HGF/SF, in astrocytic tumours, The type of cells that express c-Met in tumour tissues was also identified,Methods and results: c-Met expression was screened immunohistochemically in a total of 43 astrocytic tumours, including 14 low-grade astrocytomas (A), 13 anaplastic astrocytomas (AA) and 16 glioblastoma multiforme (GBM). c-Met reactivity was demonstrated predominantly in the cytoplasm of tumour cells, Bizarre large tumour cells tended to stain intensely. Higher c-Met expression levels (greater than or equal to 2+, more than 25 % cells were positive) were noted in 21.4%, of (A) vs. 53.8% in (AA) and 87.5% in (GBM) (P < 0.001), indicating a dear relationship between c-Met protein staining and higher grade astrocytic tumours, Moreover, c-Met immunoreactivity was also shown in tumour micro microvasculature. reactive astrocytes, and neurones in the cortex infiltrated by glioma cells. In 85.7% of cases containing infiltrated cortex, neurones were positive vs, no neurones in non-neoplastic regions (P < 0.002).Conclusions: This evidence suggests that c-Met expression in the brain could be associated with astrocytoma progression and also reactive process. Immunohistochemical determination of c-Met-expressing cell types helps to understand possible roles of c-Met in tumour tissues.