Early gene expression events in ferrets in response to SARS coronavirus infection versus direct interferon-alpha2b stimulation.
Early gene expression events in ferrets in response to SARS coronavirus infection versus direct interferon-alpha2b stimulation.
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DOI：
10.1016/j.virol.2010.10.002
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发表时间：
2011-01-05
期刊：
影响因子：
3.7
通讯作者：
Kelvin DJ
中科院分区：
文献类型：
--
作者：
Danesh A;Cameron CM;León AJ;Ran L;Xu L;Fang Y;Kelvin AA;Rowe T;Chen H;Guan Y;Jonsson CB;Cameron MJ;Kelvin DJ
I型干扰素(IFN)对病毒疾病的清除至关重要，然而，病毒与细胞直接相互作用上调的基因和二次产生干扰素上调的基因之间并没有明显的区别。在这里，我们研究了皮下注射干扰素-α2b和感染SARS冠状病毒期间雪貂的差异基因调控。体内实验表明，干扰素-α2b导致STAT1磷酸化和上调丰富的干扰素反应基因、趋化因子受体和其他参与吞噬和白细胞跨内皮细胞迁移的基因。在感染SARS-CoV期间，不仅各种IRG上调，而且参与细胞迁移和炎症的基因范围也明显更广。这项工作允许解剖SARS冠状病毒期间存在的几个分子特征，这些特征是强大的干扰素抗病毒反应的一部分。这些特征可作为评估病毒感染期间干扰素反应状态和不同病毒特定特征的有用标记物。
Type I interferons (IFNs) are essential to the clearance of viral diseases, however, a clear distinction between genes upregulated by direct virus–cell interactions and genes upregulated by secondary IFN production has not been made. Here, we investigated differential gene regulation in ferrets upon subcutaneous administration of IFN-α2b and during SARS-CoV infection. In vivo experiments revealed that IFN-α2b causes STAT1 phosphorylation and upregulation of abundant IFN response genes (IRGs), chemokine receptors, and other genes that participate in phagocytosis and leukocyte transendothelial migration. During infection with SARS-CoV not only a variety of IRGs were upregulated, but also a significantly broader range of genes involved in cell migration and inflammation. This work allowed dissection of several molecular signatures present during SARS-CoV which are part of a robust IFN antiviral response. These signatures can be useful markers to evaluate the status of IFN responses during a viral infection and specific features of different viruses.
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影响因子：
3.7
作者：
Rowe T;León AJ;Crevar CJ;Carter DM;Xu L;Ran L;Fang Y;Cameron CM;Cameron MJ;Banner D;Ng DC;Ran R;Weirback HK;Wiley CA;Kelvin DJ;Ross TM
通讯作者：
Ross TM
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